Optical feedback control/analysis of photoreactive euglena cells swimming in micro-aquariums

K. Ozasa*, J. Lee, S. Song, M. Hara, M. Maeda

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a two-dimensional (2D) optical feedback system to control/analyze photoreactive flagellate microbes confined in mm-scale microfluidic channels (micro-aquariums). The system uses a liquid-crystal (LC) projector to induce the phototaxis reactions of microbes, thus arbitrary 2D patterns with variable colors and intensities can be projected onto microaquariums. We demonstrate that the density and position of Euglena cells in micro-aquariums are controlled by the system dynamically and flexibly. Transient steps in the photophobic reactions of Euglena were observed with the system with a time resolution of several ten ms, showing a complex photoreaction mechanism of Euglena.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1523-1525
Number of pages3
StatePublished - 2011
Externally publishedYes
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume3

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/TerritoryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Keywords

  • Euglena gracilis
  • Flagellate microbes
  • Micro-aquariums
  • Microfluidics
  • Optical feedback control
  • Phototaxis

ASJC Scopus subject areas

  • Control and Systems Engineering

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