Optical real-time measurement of collagen denaturation

Vanitha Sankaran*, Jay Walsh

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Scopus citations


Linear birefringence is a property of collagenous tissue that results from both its composition and structure. Previous investigations have shown that birefringence provides an indication of structural changes in collagen during slow heating. We now report the birefringent response of both mature and young rat tail tendon to laser-heating. The results indicate that denaturation of collagen from mature rats induced by a 200-μs-long Ho:YAG laser pulse may not be described accurately by kinetic parameters. Several second-long pulses of CO2 laser pulse may not be described from young rats fit an Arrhenius model with Ea = 12.1 kcal/mol and A = e18.03 s-1. Typically, for slow-heating of collagen, Ea = kcal/mol and A = e120 s-1. Thus, it seems likely that the temperature and energy needed to initiate collagen denaturation is lower in young collagen, possibly due to its decreased hydroxyproline content and consequent decreased thermal stability.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Number of pages9
ISBN (Print)0819423866
StatePublished - Dec 1 1997
EventLaser-Tissue Interaction VIII - San Jose, CA, USA
Duration: Feb 9 1997Feb 12 1997


OtherLaser-Tissue Interaction VIII
CitySan Jose, CA, USA

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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