Optimization of a microfluidic device for localized electroporation of cells

Wonmo Kang, S. Shiva P. Nathamgari, Juan P. Giraldo-Vela, Rebecca L. McNaughton, John Kessler, Horacio Dante Espinosa*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

The transfection of molecules into cultured cells is a critical step toward fundamental studies of cells, drug screening, and stem cell reprogramming/differentiation. Despite rapid development of transfection methods, efficient transfection of adherent cells while maintaining viability is still challenging, in particular, for sensitive primary cells. To achieve this goal, we present a microfluidic device containing a membrane with micro-or nanochannels, which allow transfection of adherent cells by localized electroporation through the channels. We used numerical simulations to explore the design parameter space of the microfluidic device to quantify the electric field applied near a target cell as a function of the channel dimensions. This analysis predicted that the microfluidic device applies a highly focused electric field to only the small area of a cell that is in contact with the membrane channel, which is a unique feature that minimizes stress to the cell. We transfected HeLa and HT1080 cells with DNA plasmid with efficiencies up to 50% while maintaining cell viability similar to control experiments without electroporation. Thus, this microfluidic device for on-chip cell culture and localized electroporation offers a gentle, yet effective transfection method and maintains high viability.

Original languageEnglish (US)
Title of host publication14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1-3
Number of pages3
ISBN (Electronic)9781479956227
DOIs
StatePublished - Nov 26 2014
Event2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014 - Toronto, Canada
Duration: Aug 18 2014Aug 21 2014

Publication series

Name14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014

Other

Other2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014
CountryCanada
CityToronto
Period8/18/148/21/14

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Computer Science Applications
  • Modeling and Simulation
  • Instrumentation

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