TY - JOUR
T1 - Optimization of electron transfer dissociation via informed selection of reagents and operating parameters
AU - Compton, Philip D.
AU - Strukl, Joseph V.
AU - Bai, Dina L.
AU - Shabanowitz, Jeffrey
AU - Hunt, Donald F.
PY - 2012/2/7
Y1 - 2012/2/7
N2 - Electron transfer dissociation (ETD) has improved the mass spectrometric analysis of proteins and peptides with labile post-translational modifications and larger intact masses. Here, the parameters governing the reaction rate of ETD are examined experimentally. Currently, due to reagent injection and isolation events as well as longer reaction times, ETD spectra require significantly more time to acquire than collision-induced dissociation (CID) spectra (>100 ms), resulting in a trade-off in the dynamic range of tandem MS analyses when ETD-based methods are compared to CID-based methods. Through fine adjustment of reaction parameters and the selection of reagents with optimal characteristics, we demonstrate a drastic reduction in the time taken per ETD event. In fact, ETD can be performed with optimal efficiency in nearly the same time as CID at low precursor charge state (z = +3) and becomes faster at higher charge state (z > +3).
AB - Electron transfer dissociation (ETD) has improved the mass spectrometric analysis of proteins and peptides with labile post-translational modifications and larger intact masses. Here, the parameters governing the reaction rate of ETD are examined experimentally. Currently, due to reagent injection and isolation events as well as longer reaction times, ETD spectra require significantly more time to acquire than collision-induced dissociation (CID) spectra (>100 ms), resulting in a trade-off in the dynamic range of tandem MS analyses when ETD-based methods are compared to CID-based methods. Through fine adjustment of reaction parameters and the selection of reagents with optimal characteristics, we demonstrate a drastic reduction in the time taken per ETD event. In fact, ETD can be performed with optimal efficiency in nearly the same time as CID at low precursor charge state (z = +3) and becomes faster at higher charge state (z > +3).
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U2 - 10.1021/ac202807h
DO - 10.1021/ac202807h
M3 - Article
C2 - 22182179
AN - SCOPUS:84856690645
SN - 0003-2700
VL - 84
SP - 1781
EP - 1785
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 3
ER -