Optimization of Pulsed-DEER Measurements for Gd-Based Labels: Choice of Operational Frequencies, Pulse Durations and Positions, and Temperature

A. Raitsimring*, A. V. Astashkin, J. H. Enemark, I. Kaminker, D. Goldfarb, E. D. Walter, Y. Song, T. J. Meade

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

In this work, the experimental conditions and parameters necessary to optimize the long-distance (≥60 Å) double electron-electron resonance (DEER) measurements of biomacromolecules labeled with Gd(III) tags are analyzed. The specific parameters discussed are the temperature, microwave band, the separation between the pumping and observation frequencies, pulse train repetition rate, pulse durations and pulse positioning in the electron paramagnetic resonance spectrum. It was found that: (1) in optimized DEER measurements, the observation pulses have to be applied at the maximum of the electron paramagnetic resonance spectrum; (2) the optimal temperature range for Ka-band measurements is 14-17 K, while in W-band the optimal temperatures are between 6 and 9 K; (iv) W-band is preferable to Ka-band for DEER measurements. Recent achievements and the conditions necessary for short-distance measurements (<15 Å) are also briefly discussed.

Original languageEnglish (US)
Pages (from-to)649-670
Number of pages22
JournalApplied Magnetic Resonance
Volume44
Issue number6
DOIs
StatePublished - Jun 1 2013

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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