Optimization of the gene-expression by inducing lac promotor-regulated gene with lactose

Songwang Hou*, Xiaojie Li, Huiwen Ma

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The optimization of lactose-induced over expression of the cloned Escherichia coli RNA polymerase σ70 factor gene in Escherichia coli strain BL21 (DE3) was reported. It was found that the expression level of σ70 factor gene inserted on a phagemid pGEMEX, was the highest when the strain BL21 (DE3) harboring the phagemid was induced for 5 h with 0.08% of lactose at a growth level of OD600 nm = 1.0. Feeding lactose solution in a lower concentration at an interval of 30 min. into the bacterial culture at the optimal growth level could slightly promote the synthesis of σ70 factor.

Original languageEnglish (US)
JournalWuhan Daxue Xuebao/Journal of Wuhan University
Volume44
Issue number4
StatePublished - Dec 1 1998

Keywords

  • Gene expression
  • Lac promoter
  • Lactose as an inducer

ASJC Scopus subject areas

  • Management of Technology and Innovation

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