Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging

Ellen M. Quardokus, Diane C. Saunders, Elizabeth McDonough, John W. Hickey, Christopher Werlein, Christine Surrette, Presha Rajbhandari, Anna Martinez Casals, Hua Tian, Lisa Lowery, Elizabeth K. Neumann, Frida Björklund, Taruna V. Neelakantan, Josh Croteau, Anne E. Wiblin, Jeremy Fisher, April J. Livengood, Karen G. Dowell, Jonathan C. Silverstein, Jeffrey M. SpragginsGloria S. Pryhuber, Gail Deutsch, Fiona Ginty, Garry P. Nolan, Simon Melov, Danny Jonigk, Michael A. Caldwell, Ioannis S. Vlachos, Werner Muller, Nils Gehlenborg, Brent R. Stockwell, Emma Lundberg, Michael P. Snyder, Ronald N. Germain, Jeannie M. Camarillo, Neil L. Kelleher, Katy Börner, Andrea J. Radtke*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.

Original languageEnglish (US)
Pages (from-to)1174-1178
Number of pages5
JournalNature Methods
Issue number8
StatePublished - Aug 2023

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry
  • Biotechnology
  • Cell Biology


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