TY - JOUR
T1 - Ovary-specific novel peroxisome proliferator activated receptors-gamma transcripts in buffalo
AU - Sharma, Isha
AU - Monga, Rachna
AU - Singh, Natwar
AU - Datta, Tirtha Kumar
AU - Singh, Dheer
N1 - Funding Information:
We thank director of National Dairy Research Institute, Karnal, for providing necessary facilities for this work. This work was financially supported by NDRI SRF to Isha Sharma and NICHE grant of Indian Council of Agriculture Research (ICAR), India.
PY - 2012/8/10
Y1 - 2012/8/10
N2 - In the present study, we describe the isolation and characterization of the transcripts encoding peroxisome proliferator-activated receptor gamma (PPARγ1 and PPARγ2) in buffalo ovary. 5' RACE experiments and sequence analysis showed that these transcripts (PPARγ1a, PPARγ1b and PPARγ2) were transcribed by the different promoter usage and alternative splicing of terminal 5'-exon. The distribution of these isoforms of PPARγ transcripts in different tissues (ovary, mammary gland, spleen, liver, lung, adipose tissue) was investigated using quantitative real time analysis. Tissue- and transcript-specific expression analyses showed that a transcript, transcribed from distal promoter, not only expressed preferentially in ovary but contributes predominantly to PPAR gamma expression in ovary. Western blot analysis of both, in vivo and in vitro, experiments also supported that PPARγ1 predominantly expressed in ovary. In buffalo granulosa cells culture, the isolated transcripts were found to be up-regulated by both natural (CLA) and synthetic (Rosiglitazone) ligands and effect was reversed by PPARγ antagonist GW9662. In conclusion, the present study identified an ovary-specific novel transcript, transcribed by distal promoter, predominantly expressed in ovary which could have functional relevance in buffalo ovary.
AB - In the present study, we describe the isolation and characterization of the transcripts encoding peroxisome proliferator-activated receptor gamma (PPARγ1 and PPARγ2) in buffalo ovary. 5' RACE experiments and sequence analysis showed that these transcripts (PPARγ1a, PPARγ1b and PPARγ2) were transcribed by the different promoter usage and alternative splicing of terminal 5'-exon. The distribution of these isoforms of PPARγ transcripts in different tissues (ovary, mammary gland, spleen, liver, lung, adipose tissue) was investigated using quantitative real time analysis. Tissue- and transcript-specific expression analyses showed that a transcript, transcribed from distal promoter, not only expressed preferentially in ovary but contributes predominantly to PPAR gamma expression in ovary. Western blot analysis of both, in vivo and in vitro, experiments also supported that PPARγ1 predominantly expressed in ovary. In buffalo granulosa cells culture, the isolated transcripts were found to be up-regulated by both natural (CLA) and synthetic (Rosiglitazone) ligands and effect was reversed by PPARγ antagonist GW9662. In conclusion, the present study identified an ovary-specific novel transcript, transcribed by distal promoter, predominantly expressed in ovary which could have functional relevance in buffalo ovary.
KW - Buffalo
KW - Granulosa cells
KW - Ovary
KW - PPARγ
KW - RACE
KW - Tissue-specific transcripts
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U2 - 10.1016/j.gene.2012.04.090
DO - 10.1016/j.gene.2012.04.090
M3 - Article
C2 - 22609729
AN - SCOPUS:84862759725
SN - 0378-1119
VL - 504
SP - 245
EP - 252
JO - Gene
JF - Gene
IS - 2
ER -