Overexpression, amplification, and androgen regulation of TPD52 in prostate cancer

Mark A. Rubin; Sooryanarayana Varambally; Rameen Beroukhim; Scott A. Tomlins; Daniel R. Rhodes; Pamela L. Paris; Matthias D. Hofer; Martina Storz-Schweizer; Rainer Kuefer; Jonathan A. Fletcher; Bae Li Hsi; Jennifier A. Byrne; Kenneth J. Pienta; Colin Collins; William R. Sellers; Arul M. Chinnaiyan

doi:10.1158/0008-5472.CAN-03-3881

Overexpression, amplification, and androgen regulation of TPD52 in prostate cancer

Mark A. Rubin^*, Sooryanarayana Varambally, Rameen Beroukhim, Scott A. Tomlins, Daniel R. Rhodes, Pamela L. Paris, Matthias D. Hofer, Martina Storz-Schweizer, Rainer Kuefer, Jonathan A. Fletcher, Bae Li Hsi, Jennifier A. Byrne, Kenneth J. Pienta, Colin Collins, William R. Sellers, Arul M. Chinnaiyan

^*Corresponding author for this work

Urology

Research output: Contribution to journal › Article › peer-review

140 Scopus citations

Abstract

Gains in the long arm of chromosome 8 (8q) are believed to be associated with poor outcome and the development of hormone-refractory prostate cancer. Based on a meta-analysis of gene expression microarray data from multiple prostate cancer studies (D. R. Rhodes et al., Cancer Res 2002;62:4427-33), a candidate oncogene, Tumor Protein D52 (TPD52), was identified in the 8q21 amplicon. TPD52 is a coiled-coil motif-bearing protein, potentially involved in vesicle trafficking. Both mRNA and protein levels of TPD52 were highly elevated in prostate cancer tissues. Array comparative genomic hybridization and amplification analysis using single nucleotide polymorphism arrays demonstrated increased DNA copy number in the region encompassing TPD52. Fluorescence in situ hybridization on tissue microarrays confirmed TPD52 amplification in prostate cancer epithelia. Furthermore, our studies suggest that TPD52 protein levels may be regulated by androgens, consistent with the presence of androgen response elements in the upstream promoter of TPD52. In summary, these findings suggest that dysregulation of TPD52 by genomic amplification and androgen induction may play a role in prostate cancer progression.

Original language	English (US)
Pages (from-to)	3814-3822
Number of pages	9
Journal	Cancer Research
Volume	64
Issue number	11
DOIs	https://doi.org/10.1158/0008-5472.CAN-03-3881
State	Published - Jun 1 2004

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1158/0008-5472.CAN-03-3881

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Rubin, M. A., Varambally, S., Beroukhim, R., Tomlins, S. A., Rhodes, D. R., Paris, P. L., Hofer, M. D., Storz-Schweizer, M., Kuefer, R., Fletcher, J. A., Hsi, B. L., Byrne, J. A., Pienta, K. J., Collins, C., Sellers, W. R., & Chinnaiyan, A. M. (2004). Overexpression, amplification, and androgen regulation of TPD52 in prostate cancer. Cancer Research, 64(11), 3814-3822. https://doi.org/10.1158/0008-5472.CAN-03-3881

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title = "Overexpression, amplification, and androgen regulation of TPD52 in prostate cancer",

abstract = "Gains in the long arm of chromosome 8 (8q) are believed to be associated with poor outcome and the development of hormone-refractory prostate cancer. Based on a meta-analysis of gene expression microarray data from multiple prostate cancer studies (D. R. Rhodes et al., Cancer Res 2002;62:4427-33), a candidate oncogene, Tumor Protein D52 (TPD52), was identified in the 8q21 amplicon. TPD52 is a coiled-coil motif-bearing protein, potentially involved in vesicle trafficking. Both mRNA and protein levels of TPD52 were highly elevated in prostate cancer tissues. Array comparative genomic hybridization and amplification analysis using single nucleotide polymorphism arrays demonstrated increased DNA copy number in the region encompassing TPD52. Fluorescence in situ hybridization on tissue microarrays confirmed TPD52 amplification in prostate cancer epithelia. Furthermore, our studies suggest that TPD52 protein levels may be regulated by androgens, consistent with the presence of androgen response elements in the upstream promoter of TPD52. In summary, these findings suggest that dysregulation of TPD52 by genomic amplification and androgen induction may play a role in prostate cancer progression.",

author = "Rubin, {Mark A.} and Sooryanarayana Varambally and Rameen Beroukhim and Tomlins, {Scott A.} and Rhodes, {Daniel R.} and Paris, {Pamela L.} and Hofer, {Matthias D.} and Martina Storz-Schweizer and Rainer Kuefer and Fletcher, {Jonathan A.} and Hsi, {Bae Li} and Byrne, {Jennifier A.} and Pienta, {Kenneth J.} and Colin Collins and Sellers, {William R.} and Chinnaiyan, {Arul M.}",

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doi = "10.1158/0008-5472.CAN-03-3881",

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Rubin, MA, Varambally, S, Beroukhim, R, Tomlins, SA, Rhodes, DR, Paris, PL, Hofer, MD, Storz-Schweizer, M, Kuefer, R, Fletcher, JA, Hsi, BL, Byrne, JA, Pienta, KJ, Collins, C, Sellers, WR & Chinnaiyan, AM 2004, 'Overexpression, amplification, and androgen regulation of TPD52 in prostate cancer', Cancer Research, vol. 64, no. 11, pp. 3814-3822. https://doi.org/10.1158/0008-5472.CAN-03-3881

TY - JOUR

T1 - Overexpression, amplification, and androgen regulation of TPD52 in prostate cancer

AU - Rubin, Mark A.

AU - Varambally, Sooryanarayana

AU - Beroukhim, Rameen

AU - Tomlins, Scott A.

AU - Rhodes, Daniel R.

AU - Paris, Pamela L.

AU - Hofer, Matthias D.

AU - Storz-Schweizer, Martina

AU - Kuefer, Rainer

AU - Fletcher, Jonathan A.

AU - Hsi, Bae Li

AU - Byrne, Jennifier A.

AU - Pienta, Kenneth J.

AU - Collins, Colin

AU - Sellers, William R.

AU - Chinnaiyan, Arul M.

PY - 2004/6/1

Y1 - 2004/6/1

N2 - Gains in the long arm of chromosome 8 (8q) are believed to be associated with poor outcome and the development of hormone-refractory prostate cancer. Based on a meta-analysis of gene expression microarray data from multiple prostate cancer studies (D. R. Rhodes et al., Cancer Res 2002;62:4427-33), a candidate oncogene, Tumor Protein D52 (TPD52), was identified in the 8q21 amplicon. TPD52 is a coiled-coil motif-bearing protein, potentially involved in vesicle trafficking. Both mRNA and protein levels of TPD52 were highly elevated in prostate cancer tissues. Array comparative genomic hybridization and amplification analysis using single nucleotide polymorphism arrays demonstrated increased DNA copy number in the region encompassing TPD52. Fluorescence in situ hybridization on tissue microarrays confirmed TPD52 amplification in prostate cancer epithelia. Furthermore, our studies suggest that TPD52 protein levels may be regulated by androgens, consistent with the presence of androgen response elements in the upstream promoter of TPD52. In summary, these findings suggest that dysregulation of TPD52 by genomic amplification and androgen induction may play a role in prostate cancer progression.

AB - Gains in the long arm of chromosome 8 (8q) are believed to be associated with poor outcome and the development of hormone-refractory prostate cancer. Based on a meta-analysis of gene expression microarray data from multiple prostate cancer studies (D. R. Rhodes et al., Cancer Res 2002;62:4427-33), a candidate oncogene, Tumor Protein D52 (TPD52), was identified in the 8q21 amplicon. TPD52 is a coiled-coil motif-bearing protein, potentially involved in vesicle trafficking. Both mRNA and protein levels of TPD52 were highly elevated in prostate cancer tissues. Array comparative genomic hybridization and amplification analysis using single nucleotide polymorphism arrays demonstrated increased DNA copy number in the region encompassing TPD52. Fluorescence in situ hybridization on tissue microarrays confirmed TPD52 amplification in prostate cancer epithelia. Furthermore, our studies suggest that TPD52 protein levels may be regulated by androgens, consistent with the presence of androgen response elements in the upstream promoter of TPD52. In summary, these findings suggest that dysregulation of TPD52 by genomic amplification and androgen induction may play a role in prostate cancer progression.

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U2 - 10.1158/0008-5472.CAN-03-3881

DO - 10.1158/0008-5472.CAN-03-3881

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C2 - 15172988

AN - SCOPUS:2542594616

SN - 0008-5472

VL - 64

SP - 3814

EP - 3822

JO - Cancer Research

JF - Cancer Research

IS - 11

ER -

Overexpression, amplification, and androgen regulation of TPD52 in prostate cancer

Abstract

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