Overexpression of AQP5, a putative oncogene, promotes cell growth and transformation

Janghee Woo; Juna Lee; Young Kwang Chae; Myoung Sook Kim; Jin Hyen Baek; Jong Chul Park; Min Joo Park; Ian M. Smith; Barry Trink; Edward Ratovitski; Taekyul Lee; Bumsoo Park; Se Jin Jang; Jean C. Soria; Joseph A. Califano; David Sidransky; Chulso Moon

doi:10.1016/j.canlet.2008.01.029

Overexpression of AQP5, a putative oncogene, promotes cell growth and transformation

Janghee Woo, Juna Lee, Young Kwang Chae, Myoung Sook Kim, Jin Hyen Baek, Jong Chul Park, Min Joo Park, Ian M. Smith, Barry Trink, Edward Ratovitski, Taekyul Lee, Bumsoo Park, Se Jin Jang, Jean C. Soria, Joseph A. Califano, David Sidransky, Chulso Moon^*

^*Corresponding author for this work

Medicine, Hematology Oncology Division

Research output: Contribution to journal › Article › peer-review

88 Scopus citations

Abstract

Overexpression of several aquaporins has been reported in different types of human cancer but the role of AQPs in human carcinogenesis has not yet been clearly defined. Here, we demonstrate that ectopic expression of human AQP5 (hAQP5), a water channel expressed in lung, salivary glands, and kidney, induces many phenotypic changes characteristic of transformation both in vitro and in vivo. Furthermore, the cell proliferative ability of AQP5 appears to be dependent upon the phosphorylation of a cAMP-protein kinase (PKA) consensus site located in a cytoplasmic loop of AQP5. In addition, phosphorylation of the PKA consensus site was found to be phosphorylated preferentially in tumors. These findings altogether indicate that hAQP5 plays an important role in human carcinogenesis and, furthermore, provide an attractive therapeutic target.

Original language	English (US)
Pages (from-to)	54-62
Number of pages	9
Journal	Cancer Letters
Volume	264
Issue number	1
DOIs	https://doi.org/10.1016/j.canlet.2008.01.029
State	Published - Jun 8 2008

Keywords

AQP5
Aquaporins
Overexpression

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2008.01.029

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Woo, J., Lee, J., Chae, Y. K., Kim, M. S., Baek, J. H., Park, J. C., Park, M. J., Smith, I. M., Trink, B., Ratovitski, E., Lee, T., Park, B., Jang, S. J., Soria, J. C., Califano, J. A., Sidransky, D., & Moon, C. (2008). Overexpression of AQP5, a putative oncogene, promotes cell growth and transformation. Cancer Letters, 264(1), 54-62. https://doi.org/10.1016/j.canlet.2008.01.029

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title = "Overexpression of AQP5, a putative oncogene, promotes cell growth and transformation",

abstract = "Overexpression of several aquaporins has been reported in different types of human cancer but the role of AQPs in human carcinogenesis has not yet been clearly defined. Here, we demonstrate that ectopic expression of human AQP5 (hAQP5), a water channel expressed in lung, salivary glands, and kidney, induces many phenotypic changes characteristic of transformation both in vitro and in vivo. Furthermore, the cell proliferative ability of AQP5 appears to be dependent upon the phosphorylation of a cAMP-protein kinase (PKA) consensus site located in a cytoplasmic loop of AQP5. In addition, phosphorylation of the PKA consensus site was found to be phosphorylated preferentially in tumors. These findings altogether indicate that hAQP5 plays an important role in human carcinogenesis and, furthermore, provide an attractive therapeutic target.",

keywords = "AQP5, Aquaporins, Overexpression",

author = "Janghee Woo and Juna Lee and Chae, {Young Kwang} and Kim, {Myoung Sook} and Baek, {Jin Hyen} and Park, {Jong Chul} and Park, {Min Joo} and Smith, {Ian M.} and Barry Trink and Edward Ratovitski and Taekyul Lee and Bumsoo Park and Jang, {Se Jin} and Soria, {Jean C.} and Califano, {Joseph A.} and David Sidransky and Chulso Moon",

note = "Funding Information: The study was supported in part by the SPORE grant P50 CA96784-01 (to C.M.), Fondation de France, AP-HP and Lilly Fondation Grant (to J.-C.S.), Cancer Research Grant from Pyung-Ya Foundation (to C.M.), and KOSEF research grant (R01-2004-000-10670-0 to S.J.J.).",

year = "2008",

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doi = "10.1016/j.canlet.2008.01.029",

language = "English (US)",

volume = "264",

pages = "54--62",

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T1 - Overexpression of AQP5, a putative oncogene, promotes cell growth and transformation

AU - Woo, Janghee

AU - Lee, Juna

AU - Chae, Young Kwang

AU - Kim, Myoung Sook

AU - Baek, Jin Hyen

AU - Park, Jong Chul

AU - Park, Min Joo

AU - Smith, Ian M.

AU - Trink, Barry

AU - Ratovitski, Edward

AU - Lee, Taekyul

AU - Park, Bumsoo

AU - Jang, Se Jin

AU - Soria, Jean C.

AU - Califano, Joseph A.

AU - Sidransky, David

AU - Moon, Chulso

N1 - Funding Information: The study was supported in part by the SPORE grant P50 CA96784-01 (to C.M.), Fondation de France, AP-HP and Lilly Fondation Grant (to J.-C.S.), Cancer Research Grant from Pyung-Ya Foundation (to C.M.), and KOSEF research grant (R01-2004-000-10670-0 to S.J.J.).

PY - 2008/6/8

Y1 - 2008/6/8

N2 - Overexpression of several aquaporins has been reported in different types of human cancer but the role of AQPs in human carcinogenesis has not yet been clearly defined. Here, we demonstrate that ectopic expression of human AQP5 (hAQP5), a water channel expressed in lung, salivary glands, and kidney, induces many phenotypic changes characteristic of transformation both in vitro and in vivo. Furthermore, the cell proliferative ability of AQP5 appears to be dependent upon the phosphorylation of a cAMP-protein kinase (PKA) consensus site located in a cytoplasmic loop of AQP5. In addition, phosphorylation of the PKA consensus site was found to be phosphorylated preferentially in tumors. These findings altogether indicate that hAQP5 plays an important role in human carcinogenesis and, furthermore, provide an attractive therapeutic target.

AB - Overexpression of several aquaporins has been reported in different types of human cancer but the role of AQPs in human carcinogenesis has not yet been clearly defined. Here, we demonstrate that ectopic expression of human AQP5 (hAQP5), a water channel expressed in lung, salivary glands, and kidney, induces many phenotypic changes characteristic of transformation both in vitro and in vivo. Furthermore, the cell proliferative ability of AQP5 appears to be dependent upon the phosphorylation of a cAMP-protein kinase (PKA) consensus site located in a cytoplasmic loop of AQP5. In addition, phosphorylation of the PKA consensus site was found to be phosphorylated preferentially in tumors. These findings altogether indicate that hAQP5 plays an important role in human carcinogenesis and, furthermore, provide an attractive therapeutic target.

KW - AQP5

KW - Aquaporins

KW - Overexpression

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AN - SCOPUS:42649136174

SN - 0304-3835

VL - 264

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JO - Cancer Letters

JF - Cancer Letters

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ER -

Overexpression of AQP5, a putative oncogene, promotes cell growth and transformation

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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