Overexpression of Exportin 5 enhances RNA interference mediated by short hairpin RNAs and microRNAs

Rui Yi, Brian P. Doehle, Yi Qin, Ian G. Macara, Bryan R. Cullen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

192 Scopus citations

Abstract

Plasmids or viral vectors that express short hairpin RNAs (shRNAs) have emerged as important tools for the stable inhibition of specific genes by RNA interference. shRNAs are structural and functional homologs of pre-microRNAs, intermediates in the production of endogenously encoded microRNAs (miRNAs). Therefore, overexpressed shRNAs could inhibit miRNA function by competing for a limiting level of one or more factors involved in miRNA biogenesis or function. Here, we demonstrate that overexpressed shRNAs can saturate the activity of endogenous Exportin 5, a factor required for nuclear export of both shRNAs and pre-miRNAs. While shRNA overexpression can therefore inhibit miRNA function, simultaneous overexpression of Exportin 5 reverses this effect. Moreover, Exportin 5 overexpression can significantly enhance RNA interference mediated by shRNAs. These data have implications for the future clinical utilization of shRNAs and also provide a simple method to enhance RNA interference by shRNAs in culture.

Original languageEnglish (US)
Pages (from-to)220-226
Number of pages7
JournalRNA
Volume11
Issue number2
DOIs
StatePublished - Feb 2005
Externally publishedYes

Keywords

  • MicroRNAs
  • Nuclear RNA export
  • RNA interference

ASJC Scopus subject areas

  • Molecular Biology

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