Overexpression of fucosyltransferase IV in A431 cell line increases cell proliferation

Xuesong Yang, Zhenbo Zhang, Shuang Jia, Yuejian Liu, Xiao-Qi Wang, Qiu Yan*

*Corresponding author for this work

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Fucosyltransferase IV is an essential enzyme that catalyzes the synthesis of fucosylated oligosaccharides by transferring GDP-fucose to the terminal N-acetylglucosamine with the α1,3-linkage. Lewis Y oligosaccharide has a terminal α1,3-linked fucose residue and elevation of Lewis Y level is seen in many epithelial cancers. The mechanism of Lewis Y elevation in neoplastic cells is still largely unknown. To study the impact of fucosyltransferase IV on Lewis Y expression and its role on neoplastic cell proliferation, a pEGFP-N1-FUT4 recombinant plasmid was developed and stably transfected into A431 cells. We found that fucosyltransferase IV overexpression promoted cell proliferation and increased the expression of proliferating cell nuclear antigen that correlated with Lewis Y augmentation. Cell cycle analysis demonstrated that fucosyltransferase IV overexpression facilitated cell cycle progression. In conclusion, fucosyltransferase IV overexpression augments Lewis Y expression to trigger neoplastic cell proliferation. These studies suggest that fucosyltransferase IV may serve as a potential therapeutic target for the treatment of Lewis Y-positive epithelial cancers.

Original languageEnglish (US)
Pages (from-to)1722-1730
Number of pages9
JournalInternational Journal of Biochemistry and Cell Biology
Volume39
Issue number9
DOIs
StatePublished - Jul 26 2007

Keywords

  • Cell cycle
  • Fucosyltransferase IV
  • Gene transfection
  • Lewis antigen
  • Proliferation

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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