TY - JOUR
T1 - Overexpression of glutathione S-transferase α in clear cell renal cell carcinoma
AU - Chuang, Shang Tian
AU - Chu, Peiguo
AU - Sugimura, Jun
AU - Tretiakova, Maria S.
AU - Papavero, Veronica
AU - Wang, Kim
AU - Tan, Minhan
AU - Lin, Fan
AU - Teh, Bin T.
AU - Yang, Ximing J.
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2005/3
Y1 - 2005/3
N2 - To determine its diagnostic value, we evaluated glutathione S-transferase α (GST-α) expression in a large number of renal cell carcinomas (RCCs). GST-α messenger RNA (mRNA) levels from 70 renal neoplasms were analyzed with complementary DNA (cDNA) microarray chips containing 21,632 cDNA clones. Furthermore, 348 primary renal tumors and 24 metastatic RCCs were subjected to immunohistochemical analysis with a GST-α-specific antibody. GST-α mRNA was elevated significantly (11.4-fold) in a majority of clear cell RCCs (28/43 [65.1%]; 28/39 [71.8%] with adjustments for informative spots) compared with other kidney tumors (1/27 [3.7%]). Strong and diffuse GST-α immunoreactivity was demonstrated in a majority of clear cell (166/202 [82.2%]; mean intensity, 2.41) and metastatic clear cell RCCs (17/24 [70.8%]; mean intensity, 2.62). Other renal tumor types did not exhibit significant GST-α immunoreactivity, confirming mRNA results. Through cDNA microarrays and immunohistochemical analysis, we demonstrated GST-α as a biomarker for clear cell RCCs.
AB - To determine its diagnostic value, we evaluated glutathione S-transferase α (GST-α) expression in a large number of renal cell carcinomas (RCCs). GST-α messenger RNA (mRNA) levels from 70 renal neoplasms were analyzed with complementary DNA (cDNA) microarray chips containing 21,632 cDNA clones. Furthermore, 348 primary renal tumors and 24 metastatic RCCs were subjected to immunohistochemical analysis with a GST-α-specific antibody. GST-α mRNA was elevated significantly (11.4-fold) in a majority of clear cell RCCs (28/43 [65.1%]; 28/39 [71.8%] with adjustments for informative spots) compared with other kidney tumors (1/27 [3.7%]). Strong and diffuse GST-α immunoreactivity was demonstrated in a majority of clear cell (166/202 [82.2%]; mean intensity, 2.41) and metastatic clear cell RCCs (17/24 [70.8%]; mean intensity, 2.62). Other renal tumor types did not exhibit significant GST-α immunoreactivity, confirming mRNA results. Through cDNA microarrays and immunohistochemical analysis, we demonstrated GST-α as a biomarker for clear cell RCCs.
KW - Complementary DNA micro-array
KW - GST-α
KW - Glutathione S-transferase α
KW - Immunohistochemistry
KW - Renal cell carcinoma
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U2 - 10.1309/AQXR6B2QPUGD638C
DO - 10.1309/AQXR6B2QPUGD638C
M3 - Article
C2 - 15716239
AN - SCOPUS:20044364767
SN - 0002-9173
VL - 123
SP - 421
EP - 429
JO - American journal of clinical pathology
JF - American journal of clinical pathology
IS - 3
ER -
