To determine its diagnostic value, we evaluated glutathione S-transferase α (GST-α) expression in a large number of renal cell carcinomas (RCCs). GST-α messenger RNA (mRNA) levels from 70 renal neoplasms were analyzed with complementary DNA (cDNA) microarray chips containing 21,632 cDNA clones. Furthermore, 348 primary renal tumors and 24 metastatic RCCs were subjected to immunohistochemical analysis with a GST-α-specific antibody. GST-α mRNA was elevated significantly (11.4-fold) in a majority of clear cell RCCs (28/43 [65.1%]; 28/39 [71.8%] with adjustments for informative spots) compared with other kidney tumors (1/27 [3.7%]). Strong and diffuse GST-α immunoreactivity was demonstrated in a majority of clear cell (166/202 [82.2%]; mean intensity, 2.41) and metastatic clear cell RCCs (17/24 [70.8%]; mean intensity, 2.62). Other renal tumor types did not exhibit significant GST-α immunoreactivity, confirming mRNA results. Through cDNA microarrays and immunohistochemical analysis, we demonstrated GST-α as a biomarker for clear cell RCCs.
- Complementary DNA micro-array
- Glutathione S-transferase α
- Renal cell carcinoma
ASJC Scopus subject areas
- Pathology and Forensic Medicine