Overexpression of glutathione S-transferase α in clear cell renal cell carcinoma

Shang Tian Chuang, Peiguo Chu, Jun Sugimura, Maria S. Tretiakova, Veronica Papavero, Kim Wang, Minhan Tan, Fan Lin, Bin T. Teh, Ximing J. Yang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

To determine its diagnostic value, we evaluated glutathione S-transferase α (GST-α) expression in a large number of renal cell carcinomas (RCCs). GST-α messenger RNA (mRNA) levels from 70 renal neoplasms were analyzed with complementary DNA (cDNA) microarray chips containing 21,632 cDNA clones. Furthermore, 348 primary renal tumors and 24 metastatic RCCs were subjected to immunohistochemical analysis with a GST-α-specific antibody. GST-α mRNA was elevated significantly (11.4-fold) in a majority of clear cell RCCs (28/43 [65.1%]; 28/39 [71.8%] with adjustments for informative spots) compared with other kidney tumors (1/27 [3.7%]). Strong and diffuse GST-α immunoreactivity was demonstrated in a majority of clear cell (166/202 [82.2%]; mean intensity, 2.41) and metastatic clear cell RCCs (17/24 [70.8%]; mean intensity, 2.62). Other renal tumor types did not exhibit significant GST-α immunoreactivity, confirming mRNA results. Through cDNA microarrays and immunohistochemical analysis, we demonstrated GST-α as a biomarker for clear cell RCCs.

Original languageEnglish (US)
Pages (from-to)421-429
Number of pages9
JournalAmerican journal of clinical pathology
Volume123
Issue number3
DOIs
StatePublished - Mar 2005

Keywords

  • Complementary DNA micro-array
  • GST-α
  • Glutathione S-transferase α
  • Immunohistochemistry
  • Renal cell carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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