Overexpression of human glutathione S-transferase π protects NIH 3T3 cells against (±)anti BPDE cytotoxicity but not tumor formation

D. M. Boucher, P. M. Iannaccone*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

In order to explore the protective function of human glutathione S-transferase π (GST-π) in vitro and in vivo, transfected NIH 3T3 clones were examined in cytotoxicity assays using the carcinogen (±)anti-benzo(a)pyrene 7,8-diol- 9,10-epoxide (BPDE) or inoculated into nude mice and treated with the car cinogen benzo(a)pyrene (BP) to induce tumor formation. The human GST-π cDNA under the control of the murine α2(I)collagen promoter was transfected into NIH 3T3 cells and G418 resistant clones were analyzed by Southern, Northern, Western, and two-dimensional analysis. Clone A2 stably expressed human GST-π and has 2.5-fold greater activity toward the substrate 1-chloro-2,4-dinitrobenzene and a 1.7-fold increase in LD50 for BPDE in vitro when compared to control-transfected clone G3. This increase in protection, however, did not prevent the formation of BP-induced tumors in vivo.

Original languageEnglish (US)
Pages (from-to)197-203
Number of pages7
JournalPathobiology
Volume63
Issue number4
DOIs
StatePublished - Jan 1 1995

Keywords

  • Benzo(a)pyrene
  • Carcinogenesis
  • Glutathione S-transferase
  • NIH 3T3
  • Overexpression

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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