Phospholipase C-γ1 (PLC-γ1) mediates signals from various extracellular origins to evoke cellular events such as mitogenesis. Previously, we reported that PLC-γ1 was highly expressed in colorectal cancer and familial adenomatous polyposis, suggesting that PLC-γ1 might be oncogenic. In this study, we have established rat 3Y1 fibroblasts that overexpress whole PLC-γ1 and src homology 2 (SH2)-SH2-SH3 domain of PLC- γ1. These cells showed a transformed phenotype and were tumorigenic when transplanted into nude mice. These results indicate that overexpression of PLC-γ1 could transform rat fibroblasts, and the transformation is mediated by SH2-SH2-SH3 domain of PLC-γ1.
|Original language||English (US)|
|Number of pages||4|
|State||Published - Dec 15 1997|
ASJC Scopus subject areas
- Cancer Research