Overexpression of PPARγ induces adipogenic steatosis in mouse primary hepatocytes

Liang Bai, Yali Zhang, Chen Xie, Rong Wang, Sihai Zhao, Yuzhi Jia, Enqi Liu

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

OBJECTIVE: To investigate the effects of PPARγ overexpression on steatosis in mouse primary hepatocytes.

METHODS: Primary hepatocytes isolated from C57BL/6J mice were infected with either Ad/LacZ or Ad/PPARγ for 48 h. Steatosis of the primary hepatocytes was checked by Oil Red O staining. The mRNA and protein expression of adipocyte-specific genes PPARγ, aP2 and CideA were analyzed by using RT Real-time PCR and Western Blot.

RESULTS: Primary hepatocytes were small and even. Hepatocyte nuclei were round with dispersed chromatin and prominent nucleoli. Accumulated lipid droplets were observed in Ad/PPARγ-infected hepatocytes, but in Ad/LacZ-infected hepatocytes. Moreover, compared with Ad/LacZ-infected hepatocytes, the mRNA expression of PPARγ, aP2, FGF21 and CideA in Ad/PPARγ-infected hepatocytes were significantly induced, the protein expression of PPARγ and its target aP2 strongly increased.

CONCLUSION: over expression of PPARγ induces adipogenic steatosis in mouse primary hepatocytes.

Original languageEnglish (US)
Pages (from-to)68-74
Number of pages7
JournalZhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences
Volume45
Issue number1
StatePublished - Jan 1 2016
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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