Overexpression of recombinant proteins with a C-terminal thiocarboxylate: Implications for protein semisynthesis and thiamin biosynthesis

Cynthia Kinsland; Sean V. Taylor; Neil L. Kelleher; Fred W. McLafferty; Tadhg P. Begley

doi:10.1002/pro.5560070821

Overexpression of recombinant proteins with a C-terminal thiocarboxylate: Implications for protein semisynthesis and thiamin biosynthesis

Cynthia Kinsland, Sean V. Taylor, Neil L. Kelleher, Fred W. McLafferty, Tadhg P. Begley^*

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

A facile and rapid method for the production of protein C-terminal thiocarboxylates on DNA-encoded polypeptides is described. This method, which relies on the mechanism of the cleavage reaction of intein-containing fusion proteins, can produce multimilligram quantities of protein C-terminal thiocarboxylate quickly and inexpensively. The utility of this method for protein semisynthesis and implications for studies on the biosynthesis of thiamin are discussed.

Original language	English (US)
Pages (from-to)	1839-1842
Number of pages	4
Journal	Protein Science
Volume	7
Issue number	8
DOIs	https://doi.org/10.1002/pro.5560070821
State	Published - 1998

Keywords

C-terminal thiocarboxylate
Protein semisynthesis
Recombinant proteins
Thiamin biosynthesis

ASJC Scopus subject areas

Molecular Biology
Biochemistry

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10.1002/pro.5560070821

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abstract = "A facile and rapid method for the production of protein C-terminal thiocarboxylates on DNA-encoded polypeptides is described. This method, which relies on the mechanism of the cleavage reaction of intein-containing fusion proteins, can produce multimilligram quantities of protein C-terminal thiocarboxylate quickly and inexpensively. The utility of this method for protein semisynthesis and implications for studies on the biosynthesis of thiamin are discussed.",

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AU - Kinsland, Cynthia

AU - Taylor, Sean V.

AU - Kelleher, Neil L.

AU - McLafferty, Fred W.

AU - Begley, Tadhg P.

PY - 1998

Y1 - 1998

N2 - A facile and rapid method for the production of protein C-terminal thiocarboxylates on DNA-encoded polypeptides is described. This method, which relies on the mechanism of the cleavage reaction of intein-containing fusion proteins, can produce multimilligram quantities of protein C-terminal thiocarboxylate quickly and inexpensively. The utility of this method for protein semisynthesis and implications for studies on the biosynthesis of thiamin are discussed.

AB - A facile and rapid method for the production of protein C-terminal thiocarboxylates on DNA-encoded polypeptides is described. This method, which relies on the mechanism of the cleavage reaction of intein-containing fusion proteins, can produce multimilligram quantities of protein C-terminal thiocarboxylate quickly and inexpensively. The utility of this method for protein semisynthesis and implications for studies on the biosynthesis of thiamin are discussed.

KW - C-terminal thiocarboxylate

KW - Protein semisynthesis

KW - Recombinant proteins

KW - Thiamin biosynthesis

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Overexpression of recombinant proteins with a C-terminal thiocarboxylate: Implications for protein semisynthesis and thiamin biosynthesis

Abstract

Keywords

ASJC Scopus subject areas

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