Overexpression of recombinant proteins with a C-terminal thiocarboxylate: Implications for protein semisynthesis and thiamin biosynthesis

Cynthia Kinsland, Sean V. Taylor, Neil L. Kelleher, Fred W. McLafferty, Tadhg P. Begley*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

A facile and rapid method for the production of protein C-terminal thiocarboxylates on DNA-encoded polypeptides is described. This method, which relies on the mechanism of the cleavage reaction of intein-containing fusion proteins, can produce multimilligram quantities of protein C-terminal thiocarboxylate quickly and inexpensively. The utility of this method for protein semisynthesis and implications for studies on the biosynthesis of thiamin are discussed.

Original languageEnglish (US)
Pages (from-to)1839-1842
Number of pages4
JournalProtein Science
Volume7
Issue number8
DOIs
StatePublished - 1998

Keywords

  • C-terminal thiocarboxylate
  • Protein semisynthesis
  • Recombinant proteins
  • Thiamin biosynthesis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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