Overexpression of the Axl tyrosine kinase receptor in cutaneous SCC-derived cell lines and tumours

J. Green, M. Ikram, J. Vyas, N. Patel, C. M. Proby, L. Ghali, I. M. Leigh, E. A. O'Toole, A. Storey*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

The molecular mechanisms that underlie the development of squamous cell skin cancers (SSC) are poorly understood. We have used oligonucleotide microarrays to compare the differences in cellular gene expression between a series of keratinocyte cell that mimic disease progression with the aim of identifying genes that may potentially contribute towards squamous cell carcinoma (SCC) progression in vivo, and in particular to identify markers that may serve as potential therapeutic targets for SCC treatment. Gene expression differences were corroborated by polymerase chain reaction and Western blotting. We identified Axl, a receptor tyrosine kinase with transforming potential that has also been shown to have a role in cell survival, adhesion and chemotaxis, was upregulated in vitro in SCC-derived cells compared to premalignant cells. Extending the investigation to tumour biopsies showed that the Axl protein was overexpressed in vivo in a series of SCCs.

Original languageEnglish (US)
Pages (from-to)1446-1451
Number of pages6
JournalBritish Journal of Cancer
Volume94
Issue number10
DOIs
StatePublished - May 22 2006

Keywords

  • Axl
  • Receptor tyrosine kinase
  • Skin cancer

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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