Overexpression of vascular endothelial growth factor by MCF-7 breast cancer cells promotes estrogen-independent tumor growth in vivo

Ping Guo, Quan Fang, Huo Quan Tao, Christopher A. Schafer, Bruce M. Fenton, Ivan Ding, Bo Hu, Shi Yuan Cheng*

*Corresponding author for this work

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Alteration of the phenotype of breast cancers from estrogen-dependent to estrogen-independent growth often leads to the failure of antiestrogenic tumor therapies. We report that overexpression of vascular endothelial growth factor (VEGF) by estrogen-dependent MCF-7 breast cancer cells could abolish estrogen-dependent tumor growth in ovariectomized mice. In the absence of estrogen, MCF-7 VEGF-expressing tumors with increased vessel density showed growth kinetics similar to, or even greater than, that of parental MCF-7 tumors with estrogen supplementation. Overexpression of VEGF by MCF-7 cells or treatment on parental MCF-7 cells with recombinant VEGF also stimulated cell proliferation in culture. Our data suggest that VEGF stimulation of MCF-7 tumor angiogenesis and growth is mediated by both autocrine and paracrine mechanisms.

Original languageEnglish (US)
Pages (from-to)4684-4691
Number of pages8
JournalCancer Research
Volume63
Issue number15
StatePublished - Aug 1 2003

Fingerprint

Vascular Endothelial Growth Factor A
Estrogens
Breast Neoplasms
Growth
Neoplasms
MCF-7 Cells
Cell Proliferation
Phenotype

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Guo, Ping ; Fang, Quan ; Tao, Huo Quan ; Schafer, Christopher A. ; Fenton, Bruce M. ; Ding, Ivan ; Hu, Bo ; Cheng, Shi Yuan. / Overexpression of vascular endothelial growth factor by MCF-7 breast cancer cells promotes estrogen-independent tumor growth in vivo. In: Cancer Research. 2003 ; Vol. 63, No. 15. pp. 4684-4691.
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abstract = "Alteration of the phenotype of breast cancers from estrogen-dependent to estrogen-independent growth often leads to the failure of antiestrogenic tumor therapies. We report that overexpression of vascular endothelial growth factor (VEGF) by estrogen-dependent MCF-7 breast cancer cells could abolish estrogen-dependent tumor growth in ovariectomized mice. In the absence of estrogen, MCF-7 VEGF-expressing tumors with increased vessel density showed growth kinetics similar to, or even greater than, that of parental MCF-7 tumors with estrogen supplementation. Overexpression of VEGF by MCF-7 cells or treatment on parental MCF-7 cells with recombinant VEGF also stimulated cell proliferation in culture. Our data suggest that VEGF stimulation of MCF-7 tumor angiogenesis and growth is mediated by both autocrine and paracrine mechanisms.",
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Overexpression of vascular endothelial growth factor by MCF-7 breast cancer cells promotes estrogen-independent tumor growth in vivo. / Guo, Ping; Fang, Quan; Tao, Huo Quan; Schafer, Christopher A.; Fenton, Bruce M.; Ding, Ivan; Hu, Bo; Cheng, Shi Yuan.

In: Cancer Research, Vol. 63, No. 15, 01.08.2003, p. 4684-4691.

Research output: Contribution to journalArticle

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AU - Fang, Quan

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AU - Ding, Ivan

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AB - Alteration of the phenotype of breast cancers from estrogen-dependent to estrogen-independent growth often leads to the failure of antiestrogenic tumor therapies. We report that overexpression of vascular endothelial growth factor (VEGF) by estrogen-dependent MCF-7 breast cancer cells could abolish estrogen-dependent tumor growth in ovariectomized mice. In the absence of estrogen, MCF-7 VEGF-expressing tumors with increased vessel density showed growth kinetics similar to, or even greater than, that of parental MCF-7 tumors with estrogen supplementation. Overexpression of VEGF by MCF-7 cells or treatment on parental MCF-7 cells with recombinant VEGF also stimulated cell proliferation in culture. Our data suggest that VEGF stimulation of MCF-7 tumor angiogenesis and growth is mediated by both autocrine and paracrine mechanisms.

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