TY - CHAP
T1 - Overhauser Dynamic Nuclear Polarization Studies on Local Water Dynamics
AU - Kaminker, Ilia
AU - Barnes, Ryan
AU - Han, Songi
N1 - Funding Information:
We would like to thank the various members in our lab who have contributed to both improving and applying the ODNP technique, most notably John Franck, Chi-yuan Cheng, and Brandon Armstrong. In this and previous studies, we made use of the MRL Central Facilities, a member of the MRFN, supported by the NSF through the MRSEC (DMR 1121053). We also acknowledge support from the Dreyfus Teacher Scholar award to S.H., the Cluster of Excellence RESOLV (EXC 1069) funded by the Deutsche Forschungsgemeinschaft and a long-term postdoctoral fellowship by the Human Frontier Science Program awarded to I.K.
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015
Y1 - 2015
N2 - Overhauser dynamic nuclear polarization (ODNP) is an emerging technique for quantifying translational water dynamics in the vicinity (< 1 nm) of stable radicals that can be chemically attached to macromolecules of interest. This has led to many in-depth and enlightening studies of hydration water of biomolecules, revolving around the role of solvent dynamics in the structure and function of proteins, nucleic acids, and lipid bilayer membranes. Still to date, a complete and fully automated ODNP instrument is not commercialized. The purpose of this chapter is to share the technical know-how of the hardware, theory, measurement, and data analysis method needed to successfully utilize and disseminate the ODNP technique.
AB - Overhauser dynamic nuclear polarization (ODNP) is an emerging technique for quantifying translational water dynamics in the vicinity (< 1 nm) of stable radicals that can be chemically attached to macromolecules of interest. This has led to many in-depth and enlightening studies of hydration water of biomolecules, revolving around the role of solvent dynamics in the structure and function of proteins, nucleic acids, and lipid bilayer membranes. Still to date, a complete and fully automated ODNP instrument is not commercialized. The purpose of this chapter is to share the technical know-how of the hardware, theory, measurement, and data analysis method needed to successfully utilize and disseminate the ODNP technique.
KW - Hydration dynamics
KW - Overhauser dynamic nuclear polarization
KW - Protein hydration water measurements
KW - Site-directed hydration dynamics
KW - Site-directed spin labeling
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U2 - 10.1016/bs.mie.2015.06.040
DO - 10.1016/bs.mie.2015.06.040
M3 - Chapter
C2 - 26477261
AN - SCOPUS:84944352392
T3 - Methods in Enzymology
SP - 457
EP - 483
BT - Methods in Enzymology
PB - Academic Press Inc
ER -