Abstract
Overhauser dynamic nuclear polarization (ODNP) is an emerging technique for quantifying translational water dynamics in the vicinity (< 1 nm) of stable radicals that can be chemically attached to macromolecules of interest. This has led to many in-depth and enlightening studies of hydration water of biomolecules, revolving around the role of solvent dynamics in the structure and function of proteins, nucleic acids, and lipid bilayer membranes. Still to date, a complete and fully automated ODNP instrument is not commercialized. The purpose of this chapter is to share the technical know-how of the hardware, theory, measurement, and data analysis method needed to successfully utilize and disseminate the ODNP technique.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Enzymology |
| Publisher | Academic Press Inc |
| Pages | 457-483 |
| Number of pages | 27 |
| DOIs | |
| State | Published - 2015 |
Publication series
| Name | Methods in Enzymology |
|---|---|
| Volume | 564 |
| ISSN (Print) | 0076-6879 |
| ISSN (Electronic) | 1557-7988 |
Funding
We would like to thank the various members in our lab who have contributed to both improving and applying the ODNP technique, most notably John Franck, Chi-yuan Cheng, and Brandon Armstrong. In this and previous studies, we made use of the MRL Central Facilities, a member of the MRFN, supported by the NSF through the MRSEC (DMR 1121053). We also acknowledge support from the Dreyfus Teacher Scholar award to S.H., the Cluster of Excellence RESOLV (EXC 1069) funded by the Deutsche Forschungsgemeinschaft and a long-term postdoctoral fellowship by the Human Frontier Science Program awarded to I.K.
Keywords
- Hydration dynamics
- Overhauser dynamic nuclear polarization
- Protein hydration water measurements
- Site-directed hydration dynamics
- Site-directed spin labeling
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology