Overview of the role for calreticulin in the enhancement of wound healing through multiple biological effects

Leslie I. Gold*, Mohammad Rahman, Keith M. Blechman, Matthew R. Greives, Samara Churgin, Joseph Michaels, Matthew J. Callaghan, Nancy L. Cardwell, Alonda C. Pollins, Marek Michalak, John W. Siebert, Jamie P. Levine, Geoffrey C. Gurtner, Lillian B. Nanney, Robert D. Galiano, Caprice L. Cadacio

*Corresponding author for this work

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Calreticulin (CRT), an intracellular chaperone protein crucial for the proper folding and transport of proteins through the endoplasmic reticulum, has more recent acclaim as a critical regulator of extracellular functions, particularly in mediating cellular migration and as a requirement for phagocytosis of apoptotic cells. Consistent with these functions, we show that the topical application of CRT has profound effects on the process of wound healing by causing a dose-dependent increase in epithelial migration and granulation tissue formation in both murine and porcine normal and impaired animal models of skin injury. These effects of CRT are substantiated, in vitro, as we show that CRT strongly induces cell migration/wound closure of human keratinocytes and fibroblasts, using a wound/scratch plate assay, and stimulates cellular proliferation of human keratinocytes, fibroblasts, and vascular endothelial cells, providing mechanistic insight into how CRT functions in repair. Similarly, in both animal models, the histology of the wounds show marked proliferation of basal keratinocytes and dermal fibroblasts, dense cellularity of the dermis with notably increased numbers of macrophages and well-organized collagen fibril deposition. Thus, CRT profoundly affects the wound healing process by recruiting cells essential for repair into the wound, stimulating cell growth, and increasing extracellular matrix production.

Original languageEnglish (US)
Pages (from-to)57-65
Number of pages9
JournalJournal of Investigative Dermatology Symposium Proceedings
Volume11
Issue number1
DOIs
StatePublished - Sep 1 2006

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Dermatology
  • Cell Biology

Fingerprint Dive into the research topics of 'Overview of the role for calreticulin in the enhancement of wound healing through multiple biological effects'. Together they form a unique fingerprint.

  • Cite this

    Gold, L. I., Rahman, M., Blechman, K. M., Greives, M. R., Churgin, S., Michaels, J., Callaghan, M. J., Cardwell, N. L., Pollins, A. C., Michalak, M., Siebert, J. W., Levine, J. P., Gurtner, G. C., Nanney, L. B., Galiano, R. D., & Cadacio, C. L. (2006). Overview of the role for calreticulin in the enhancement of wound healing through multiple biological effects. Journal of Investigative Dermatology Symposium Proceedings, 11(1), 57-65. https://doi.org/10.1038/sj.jidsymp.5650011