Abstract
Reactive oxygen species generated by activated human phagoctyes can cause a variety of genetic injuries and produce malignant transformation in target cells. We previously reported that DNA extracted from phagocyte-transformed 10T1/2 mouse fibroblasts contained Msp I-dependent restriction fragment length polymorphisms in the c-abl oncogene. The data suggested that the oxidant-induced RFLP resulted from an alteration in the methylation pattern in c-abl. We have now mapped one of these RFLP to a specific 'CCGG' tetramer found within the tyrosine kinase region of the gene. The polymorphic 'CCGG' site has been localized to the intron between exon 2 and 3a. Restriction analysis indicates that a repetitive sequence exists within this intron and that the RFLP is associated with this repeat.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 761-769 |
| Number of pages | 9 |
| Journal | Free Radical Research Communications |
| Volume | 13 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1991 |
Funding
Supported by NIH grant IROI CA-47549. We thank Steven Satek. Patrick Turk, Robert McCarthy, and Joshua Trob for excellent technical assistance and Drs. Rex Chisholm and Preethi Gunaratne for helpful discussions.
Keywords
- Methylation c-abl
- Oxidant
- Oxygen radical
- Phagocyte
- Tyrosine kinase
ASJC Scopus subject areas
- Biochemistry