Oxidant-induced restriction polymorphism maps to kinase region of c-abl oncogene

C. J. Schmeichel*, S. A. Weitzman

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Reactive oxygen species generated by activated human phagoctyes can cause a variety of genetic injuries and produce malignant transformation in target cells. We previously reported that DNA extracted from phagocyte-transformed 10T1/2 mouse fibroblasts contained Msp I-dependent restriction fragment length polymorphisms in the c-abl oncogene. The data suggested that the oxidant-induced RFLP resulted from an alteration in the methylation pattern in c-abl. We have now mapped one of these RFLP to a specific 'CCGG' tetramer found within the tyrosine kinase region of the gene. The polymorphic 'CCGG' site has been localized to the intron between exon 2 and 3a. Restriction analysis indicates that a repetitive sequence exists within this intron and that the RFLP is associated with this repeat.

Original languageEnglish (US)
Pages (from-to)761-769
Number of pages9
JournalFree Radical Research Communications
Volume13
Issue number1
DOIs
StatePublished - Jan 1 1991

Keywords

  • Methylation c-abl
  • Oxidant
  • Oxygen radical
  • Phagocyte
  • Tyrosine kinase

ASJC Scopus subject areas

  • Biochemistry

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