Oxidative stress induced by crude venom from the jellyfish Pelagia noctiluca in neuronal-like differentiated SH-SY5Y cells

Rossana Morabito, Salvatore Condello, Monica Currò, Angela Marino, Riccardo Ientile, Giuseppina La Spada*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Marine toxins are a suitable research model and their mechanism of action is intriguing and still under debate. Either a pore formation mechanism or oxidative stress phenomena may explain the damage induced by toxins. The effect of crude venom from isolated nematocysts of the jellyfish Pelagia noctiluca on neuronal-like cells derived from human neuroblastoma SH-SY5Y has been here studied. To prove the possible oxidative stress events, cell viability, assessed by MTT quantitative colorimetric assay, intracellular reactive oxygen species (ROS) quantified by the non-fluorescent probe H2DCF-DA and changes in mitochondrial transmembrane potential (Δ Ψm) measured by the incorporation of a cationic fluorescent dye rhodamine-123 were verified on venom-treated cells (0.05-0.5 μg/ml doses). A dose- and time-dependent reduction of all parameters was observed after venom treatment. NAC (N-acetyl-cysteine), antioxidant applied before crude venom application, significantly counteracted the decrease in cell viability and ROS production, while Δ Ψm was only partially restored. The disruption of mitochondrial membrane by P. noctiluca crude venom may thus induce oxidative stress by inhibiting mitochondrial respiration and uncoupling oxidative phosphorylation, sensitizing mitochondria in SH-SY5H cells and facilitating membrane permeability. In sum, our findings suggest that P. noctiluca crude venom directly induces Δ Ψm collapse with further generation of ROS and add novel information to the understanding of such toxins, still not completely clarified.

Original languageEnglish (US)
Pages (from-to)694-699
Number of pages6
JournalToxicology in Vitro
Volume26
Issue number5
DOIs
StatePublished - Aug 1 2012

Keywords

  • Crude venom
  • Mitochondrial membrane potential
  • Nematocysts
  • Neuroblastoma cells
  • Pelagia noctiluca
  • ROS

ASJC Scopus subject areas

  • Toxicology

Fingerprint Dive into the research topics of 'Oxidative stress induced by crude venom from the jellyfish Pelagia noctiluca in neuronal-like differentiated SH-SY5Y cells'. Together they form a unique fingerprint.

Cite this