Oxygen-induced maturation of SOD1: A key role for disulfide formation by the copper chaperone CCS

Yoshiaki Furukawa, Andrew S. Torres, Thomas V. O'Halloran*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

297 Scopus citations


The antioxidant enzyme Cu,Zn-superoxide dismutase (SOD1) has the distinction of being one of the most abundant disulfide-containing protein known in the eukaryotic cytosol; however, neither catalytic nor physiological roles for the conserved disulfide are known. Here we show that the disulfide status of Saccharomyces cerevisiae SOD1 significantly affects the monomer-dimer equilibrium, the interaction with the copper chaperone CCS, and the activity of the enzyme itself. Disulfide formation in SOD1 by O2 is slow but is greatly accelerated by the Cubound form of CCS (Cu-CCS) in vivo and in vitro even in the presence of excess reductants; once formed, this disulfide is kinetically stable. Biochemical assays reveal that Cu-CCS facilitates Cys oxidation and disulfide isomerization in the stepwise conversion of the immature form of the enzyme to the active state. The immature form of SOD1 is most susceptible to oxidative insult and to aggregation reminiscent of that observed in amyotrophic lateral sclerosis. Thus Cu-CCS mediation of correct disulfide formation in SOD1 is important for regulation of enzyme activity and for prevention of misfolding or aggregation.

Original languageEnglish (US)
Pages (from-to)2872-2881
Number of pages10
JournalEMBO Journal
Issue number14
StatePublished - Jul 21 2004


  • Copper chaperone
  • Disulfide bond
  • Oxidative stress
  • Superoxide dismutase
  • fALS

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Molecular Biology
  • Neuroscience(all)


Dive into the research topics of 'Oxygen-induced maturation of SOD1: A key role for disulfide formation by the copper chaperone CCS'. Together they form a unique fingerprint.

Cite this