P element-flanked inserts at the yellow locus of Drosophila melanogaster strains

B. Kroczyńska*, A. N. Lobanov, O. P. Samarina

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Large inserts have been found to appear within the yellow (y) locus of several unstable mutant lines derived from a parental strain (y2sc+waG) of Drosophila melanogaster. The inserts of the individual mutant lines correspond roughly to a series of multimers (4.1, 7.1, 14.3 and 28.9 kbp, respectively) and are always located at the same position, somewhat upstream of the y gene transcription start site. Each of them begins and ends with a l-kbp sequence which shares restriction sites with P elements. One of these inserts (14.3 kbp) has been partially sequenced (1270 bp from the 5′ end). The first 1185 bp match exactly the sequence of an internally deleted P element. The remaining part of the sequence, on the other hand, is unrelated to P elements. It is assumed that large DNA sequences may be captured by the flanking P-element fragments and inserted into new chromosomal sites.

Original languageEnglish (US)
Pages (from-to)115-121
Number of pages7
JournalMolecular Biology Reports
Volume17
Issue number2
DOIs
StatePublished - Feb 1 1993

Keywords

  • Drosophila melanogaster
  • insertional mutation
  • P element
  • yellow locus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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