p-postlabeling analysis of peroxisome proliferator-dna adduct formation in rat liver in vivo and hepatocytes in vitro

Ramesh C. Gupta*, Sudhir K. Goel, Karen Earley, Balbir Singh, Janardan K. Reddy

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

97 Scopus citations

Abstract

Hepatocarcinogenic peroxisome proliferators, clofibrate, profibrate, Wy-14643 or di(2-ethylhexyl)phthalate, were administered once daily by gavage to groups of three male F344 rats for 3 days and the rats were killed 2 h after the last dose. The DNA isolated from the livers was analyzed for possible carcinogen-DNA adducts, by a most sensitive 32P-postlabeling technique which can detect one adduct in 1010 nucleotides. No adducts were detected by this assay in the DNA isolated from the livers of rats treated with any of the peroxisome proliferators. Adducts were also not found in the DNA of hepatocytes exposed in vitro to these peroxisome proliferators for 4 h in primary suspension cultures. Failure to detect peroxisome proliferator DNA adducts in hepatocytes under in vivo and in vitro conditions supports the contention that formation of a peroxisome proliferator-DNA adduct is not an essential step in the carcinogenesis by this novel class of carcinogens.

Original languageEnglish (US)
Pages (from-to)933-936
Number of pages4
JournalCarcinogenesis
Volume6
Issue number6
DOIs
StatePublished - Jun 1985

ASJC Scopus subject areas

  • Cancer Research

Fingerprint Dive into the research topics of 'p-postlabeling analysis of peroxisome proliferator-dna adduct formation in rat liver in vivo and hepatocytes in vitro'. Together they form a unique fingerprint.

Cite this