p130CAS is required for netrin signaling and commissural axon guidance

Guofa Liu, Weiquan Li, Xue Gao, Xiaoling Li, Claudia Jürgensen, Hwan Tae Park, Nah Young Shin, Jian Yu, Ming Liang He, Steven K. Hanks, Jane Y. Wu, Kun Liang Guan, Yi Rao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Netrins are an important family of axon guidance cues. Here, we report that netrin-1 induces tyrosine phosphorylation of p130CAS (Crk-associated substrate). Our biochemical studies indicate that p130CAS is downstream of the Src family kinases and upstream of the small GTPase Rac1 and Cdc42. Inhibition of p130CAS signaling blocks both the neurite outgrowth-promoting activity and the axon attraction activity of netrin-1. p130CAS RNA interference inhibits the attraction of commissural axons in the spinal cord by netrin-1 and causes defects in commissural axon projection in the embryo. These results demonstrate that p130CAS is a key component in the netrin signal transduction pathway and plays an important role in guiding commissural axons in vivo.

Original languageEnglish (US)
Pages (from-to)957-968
Number of pages12
JournalJournal of Neuroscience
Volume27
Issue number4
DOIs
StatePublished - Jan 24 2007

Keywords

  • Cdc42
  • Commissural axons
  • Netrin-1
  • Rac1
  • Signaling
  • p130

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint

Dive into the research topics of 'p130<sup>CAS</sup> is required for netrin signaling and commissural axon guidance'. Together they form a unique fingerprint.

Cite this