TY - JOUR
T1 - P190 RhoGAP promotes contact inhibition in epithelial cells by repressing YAP activity
AU - Frank, Scott R.
AU - Köllmann, Clemens P.
AU - Luong, Phi
AU - Galli, Giorgio G.
AU - Zou, Lihua
AU - Bernards, André
AU - Getz, Gad
AU - Calogero, Raffaele A.
AU - Frödin, Morten
AU - Hansen, Steen H.
N1 - Funding Information:
G.G. Galli was supported by an American-Italian Cancer Foundation postdoctoral research fellowship. This work used Harvard University Digestive Diseases Center core facilities and was funded by National Institutes of Health R01 CA205158 and an endowed investigatorship from the Roy and Lynne Frank Foundation (to S.H. Hansen). G. Getz is the inaugural incumbent of the Paul C. Zamecnik Chair in Oncology at the Massachusetts General Hospital Cancer Center. The authors declare no competing financial interests.
Publisher Copyright:
© 2018 Rockefeller University Press.
PY - 2018/9/1
Y1 - 2018/9/1
N2 - ARH GAP35 encoding p190A RhoGAP is a cancer-associated gene with a mutation spectrum suggestive of a tumor-suppressor function. In this study, we demonstrate that loss of heterozygosity for ARH GAP35 occurs in human tumors. We sought to identify tumor-suppressor capacities for p190A RhoGAP (p190A) and its paralog p190B in epithelial cells. We reveal an essential role for p190A and p190B to promote contact inhibition of cell proliferation (CIP), a function that relies on RhoGAP activity. Unbiased mRNA sequencing analyses establish that p190A and p190B modulate expression of genes associated with the Hippo pathway. Accordingly, we determine that p190A and p190B induce CIP by repressing YAP-TEAD-regulated gene transcription through activation of LATS kinases and inhibition of the Rho-ROCK pathway. Finally, we demonstrate that loss of a single p190 paralog is sufficient to elicit nuclear translocation of YAP and perturb CIP in epithelial cells cultured in Matrigel. Collectively, our data reveal a novel mechanism consistent with a tumor-suppressor function for ARH GAP35.
AB - ARH GAP35 encoding p190A RhoGAP is a cancer-associated gene with a mutation spectrum suggestive of a tumor-suppressor function. In this study, we demonstrate that loss of heterozygosity for ARH GAP35 occurs in human tumors. We sought to identify tumor-suppressor capacities for p190A RhoGAP (p190A) and its paralog p190B in epithelial cells. We reveal an essential role for p190A and p190B to promote contact inhibition of cell proliferation (CIP), a function that relies on RhoGAP activity. Unbiased mRNA sequencing analyses establish that p190A and p190B modulate expression of genes associated with the Hippo pathway. Accordingly, we determine that p190A and p190B induce CIP by repressing YAP-TEAD-regulated gene transcription through activation of LATS kinases and inhibition of the Rho-ROCK pathway. Finally, we demonstrate that loss of a single p190 paralog is sufficient to elicit nuclear translocation of YAP and perturb CIP in epithelial cells cultured in Matrigel. Collectively, our data reveal a novel mechanism consistent with a tumor-suppressor function for ARH GAP35.
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U2 - 10.1083/jcb.201710058
DO - 10.1083/jcb.201710058
M3 - Article
C2 - 29934311
AN - SCOPUS:85057916444
SN - 0021-9525
VL - 217
SP - 3183
EP - 3201
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 9
ER -
