p53 Gene deletions in paraffin-preserved lymphoid tumors from irradiated mice

Tatjana Paunesku, Yueru Zhang, M. Anne Gemmell, Gayle E. Woloschak*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Experiments were performed to measure deletions in the p53 gene in paraffin-embedded tissues (tumors and control) derived from mice exposed to γ-rays or neutrons up to 28 years ago. Deletions in exons 1, 3, 4, 5, 6, 7 and 9 were monitored by PCR and Southern blotting techniques. The results of these experiments demonstrated p53 deletions in only 1/6 spontaneous tumors but in 5/6 γ-ray-induced and 5/6 neutron-induced tumors. Exons deleted in tumors from γ-ray exposed mice were similar to those deleted in tumors from neutron-exposed mice. They document differences in spectra of p53 deletions in comparing spontaneous radiation-induced tumors.

Original languageEnglish (US)
Pages (from-to)511-517
Number of pages7
JournalLeukemia Research
Volume24
Issue number6
DOIs
StatePublished - Jun 2000

Keywords

  • Fission-spectrum neutrons
  • Gene deletions
  • Radiation carcinogenesis
  • Radiation effects
  • p53

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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