Abstract
Experiments were performed to measure deletions in the p53 gene in paraffin-embedded tissues (tumors and control) derived from mice exposed to γ-rays or neutrons up to 28 years ago. Deletions in exons 1, 3, 4, 5, 6, 7 and 9 were monitored by PCR and Southern blotting techniques. The results of these experiments demonstrated p53 deletions in only 1/6 spontaneous tumors but in 5/6 γ-ray-induced and 5/6 neutron-induced tumors. Exons deleted in tumors from γ-ray exposed mice were similar to those deleted in tumors from neutron-exposed mice. They document differences in spectra of p53 deletions in comparing spontaneous radiation-induced tumors.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 511-517 |
| Number of pages | 7 |
| Journal | Leukemia Research |
| Volume | 24 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 2000 |
Funding
The authors would like to thank Ms Katie Nobles for excellent secretarial service, Drs Frank Collart, Andrei Mirzabekov, Joan Bull and Aleksandar Milosavljevic for review of the manuscript, and Dr Bruce Carnes, Dr Doug Grahn and Ms B.J. Wright for help in accessing the tissues used in these studies. This work was supported by the US Department of Energy, Office of Health and Environmental Research, under contract no. W-31-109-ENG-38. T. Paunesku drafted the article, assembled and analyzed the data, provided statistical expertise and gave critical revisison. Y. Zhnag helped to assemble and interpret the data and assisted in drafting the manuscript. M.A. Gemmell helped to collect and analyze the data. G.E. Woloschak conceived and designed the study, assisted with the data interpretation, helped with the drafting and revision of the paper, obtained the funding and gave final approval.
Keywords
- Fission-spectrum neutrons
- Gene deletions
- Radiation carcinogenesis
- Radiation effects
- p53
ASJC Scopus subject areas
- Hematology
- Oncology
- Cancer Research
