P66Shc: A novel biomarker of tubular oxidative injury in patients with diabetic nephropathy

Xiaoxuan Xu, Xuejing Zhu, Mingming Ma, Yachun Han, Chun Hu, Shuguang Yuan, Yuan Yang, Li Xiao, Fuyou Liu, Yashpal S. Kanwar, Lin Sun*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Increased p66Shc expression has been associated with diabetic nephropathy (DN). However, whether p66Shc can serve as a potential biomarker for tubular oxidative injury in DN is unknown. We measured the expression of p66Shc in peripheral blood monocytes (PBMs) and renal biopsy tissues from DN patients and then analysed the relationship between p66Shc expression and the clinical characteristics of patients with DN. Patients were divided into 4 groups (class IIa, class IIb, class III and the control group). qPCR, Western blotting and immunohistochemistry were performed. The results showed that both p66Shc and p-p66Shc expression significantly increased in PBMs and kidney tissues of DN patients. Moreover, Spearman's correlation and multiple regression analyses were carried out. A positive relationship between the p66Shc expression and oxidative stress was found. p66Shc and oxidative stress were significant predictors of the degree of tubular damage. In addition, p66Shc expression was positively correlated with the concentrations of β-NAG, UACR and 8-OHdG, low-density lipoprotein and blood glucose levels, and duration of diabetes in patients with DN from class IIa to class III. These data indicated that increased expression of p66Shc may serve as a therapeutic target and a novel biomarker of DN.

Original languageEnglish (US)
Article number29302
JournalScientific reports
StatePublished - Jul 5 2016

ASJC Scopus subject areas

  • General


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