PAF and TNF increase the precursor of NF-kappa B p50 mRNA in mouse intestine: quantitative analysis by competitive PCR

Xiaodi Tan, Xiaoming Sun, Francis X. Gonzalez-Crussi, Frank Gonzalez-Crussi, Wei Hsueh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

NF-κB, a nuclear transcription factor, is involved in the regulation of inflammatory cytokines. We have previously reported that PAF and TNF induce intestinal injury in rats and mice and the interaction of TNF and PAF probably plays a central role in its pathogenesis. In the present study, we developed a competitive PCR method to quantitate the transcripts of NF-κB p50/p105 gene, and investigated the effects of PAF and TNF on p50/p105 gene expression in the small intestine of C3H/HeN mice - p105 is the precursor of the p50 subunit of NF-kB. We found that NF-κB p50/p105 gene is constitutively expressed in the normal small intestine in small quantities (7.05 ± 1.04 attomol/μg total RNA). PAF at a dose (1 μg/kg) causing no systemic changes (e.g., hypotension, hemoconcentration), markedly increased intestinal p50/p105 transcripts within 30 min. TNF, at dose (1 mg/kg) also insufficient to induce systemic changes, increased intestinal p50/p105 gene expression, although its effect was much slower than PAF. The effect of TNF was not blocked by WEB 2086, a PAF antagonist. Our results indicate that both PAF and TNF stimulate the expression of NF-κB p50/p105 in vivo. However, the mechanisms of their respective actions are probably different.

Original languageEnglish (US)
Pages (from-to)157-162
Number of pages6
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume1215
Issue number1-2
DOIs
StatePublished - Nov 17 1994

Keywords

  • Competitive PCR
  • NF-κB
  • PAF
  • Small intestine

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Endocrinology

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