PAR-3 defines a central subdomain of the cortical actin cap in mouse eggs

Francesca E. Duncan; Stuart B. Moss; Richard M. Schultz; Carmen J. Williams

doi:10.1016/j.ydbio.2004.12.034

PAR-3 defines a central subdomain of the cortical actin cap in mouse eggs

Francesca E. Duncan, Stuart B. Moss, Richard M. Schultz, Carmen J. Williams^*

^*Corresponding author for this work

Obstetrics and Gynecology

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

The evolutionarily conserved partitioning defective (PAR) protein PAR-3 is pivotal for establishing and maintaining cell polarity. During mammalian oocyte maturation, the radially symmetric oocyte is transformed into a highly polarized metaphase II (MII)-arrested egg. We therefore examined several aspects of PAR-3 expression during oocyte maturation. We cloned two novel PAR-3 transcripts from an oocyte library that likely encode proteins of M_r = 73 K and 133 K that are phosphorylated during maturation. PAR-3, which is found throughout the GV-intact oocyte, becomes asymmetrically localized during meiosis. Following germinal vesicle breakdown, PAR-3 surrounds the condensing chromosomes and associates with the meiotic spindles. Prior to emission of the first and second polar bodies, PAR-3 is located within a central subdomain of the polarized actin cap, which overlies the spindle. This cortical PAR-3 localization depends on intact microfilaments. These results suggest a role for PAR-3 in establishing asymmetry in the egg and in defining the future site of polar body emission.

Original language	English (US)
Pages (from-to)	38-47
Number of pages	10
Journal	Developmental Biology
Volume	280
Issue number	1
DOIs	https://doi.org/10.1016/j.ydbio.2004.12.034
State	Published - Apr 1 2005

Keywords

Cortical actin
Meiosis
Mouse egg
PAR-3

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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title = "PAR-3 defines a central subdomain of the cortical actin cap in mouse eggs",

abstract = "The evolutionarily conserved partitioning defective (PAR) protein PAR-3 is pivotal for establishing and maintaining cell polarity. During mammalian oocyte maturation, the radially symmetric oocyte is transformed into a highly polarized metaphase II (MII)-arrested egg. We therefore examined several aspects of PAR-3 expression during oocyte maturation. We cloned two novel PAR-3 transcripts from an oocyte library that likely encode proteins of Mr = 73 K and 133 K that are phosphorylated during maturation. PAR-3, which is found throughout the GV-intact oocyte, becomes asymmetrically localized during meiosis. Following germinal vesicle breakdown, PAR-3 surrounds the condensing chromosomes and associates with the meiotic spindles. Prior to emission of the first and second polar bodies, PAR-3 is located within a central subdomain of the polarized actin cap, which overlies the spindle. This cortical PAR-3 localization depends on intact microfilaments. These results suggest a role for PAR-3 in establishing asymmetry in the egg and in defining the future site of polar body emission.",

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note = "Funding Information: This research was supported by grants from the NIH (HD22732 to R.M.S. and C.J.W. and HD06274 to S.B.M.). F.D. was supported by the National Science Foundation Graduate Research Fellowship. We thank Teri Ord and Kerry Krauss for technical assistance and advice. The oocyte cDNA library was a generous gift from John J. Eppig and the C2-2 antibody was a generous gift from S. Ohno. Portions of this work are being submitted by F.D. in partial fulfillment for the Ph.D. requirements at the University of Pennsylvania.",

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N2 - The evolutionarily conserved partitioning defective (PAR) protein PAR-3 is pivotal for establishing and maintaining cell polarity. During mammalian oocyte maturation, the radially symmetric oocyte is transformed into a highly polarized metaphase II (MII)-arrested egg. We therefore examined several aspects of PAR-3 expression during oocyte maturation. We cloned two novel PAR-3 transcripts from an oocyte library that likely encode proteins of Mr = 73 K and 133 K that are phosphorylated during maturation. PAR-3, which is found throughout the GV-intact oocyte, becomes asymmetrically localized during meiosis. Following germinal vesicle breakdown, PAR-3 surrounds the condensing chromosomes and associates with the meiotic spindles. Prior to emission of the first and second polar bodies, PAR-3 is located within a central subdomain of the polarized actin cap, which overlies the spindle. This cortical PAR-3 localization depends on intact microfilaments. These results suggest a role for PAR-3 in establishing asymmetry in the egg and in defining the future site of polar body emission.

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PAR-3 defines a central subdomain of the cortical actin cap in mouse eggs

Abstract

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