Parallel protein purification

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Structural genomics has increased demands on protein production laboratories to supply large numbers of different proteins in high quantities and qualities in shorter times. Extensive experience with many different technologies and strategies developed and implemented by structural genomics groups have helped identify an optimal approach to purification of a large number of widely different proteins. This standardized protocol can be applied to many samples and can be used in parallel protein purification.

Original languageEnglish (US)
Pages (from-to)137-143
Number of pages7
JournalMethods in Molecular Biology
Volume1140
DOIs
StatePublished - Jan 1 2014

Keywords

  • Gel filtration
  • His-tag affinity purification
  • His-tag cleavage
  • Ion exchange chromatography
  • Parallel protein purification
  • TEV protease

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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