Parallel protein purification

Ludmilla Shuvalova

doi:10.1007/978-1-4939-0354-2_10

Parallel protein purification

Ludmilla Shuvalova

Pharmacology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Structural genomics has increased demands on protein production laboratories to supply large numbers of different proteins in high quantities and qualities in shorter times. Extensive experience with many different technologies and strategies developed and implemented by structural genomics groups have helped identify an optimal approach to purification of a large number of widely different proteins. This standardized protocol can be applied to many samples and can be used in parallel protein purification.

Original language	English (US)
Pages (from-to)	137-143
Number of pages	7
Journal	Methods in Molecular Biology
Volume	1140
DOIs	https://doi.org/10.1007/978-1-4939-0354-2_10
State	Published - 2014

Keywords

Gel filtration
His-tag affinity purification
His-tag cleavage
Ion exchange chromatography
Parallel protein purification
TEV protease

ASJC Scopus subject areas

Genetics
Molecular Biology

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10.1007/978-1-4939-0354-2_10

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abstract = "Structural genomics has increased demands on protein production laboratories to supply large numbers of different proteins in high quantities and qualities in shorter times. Extensive experience with many different technologies and strategies developed and implemented by structural genomics groups have helped identify an optimal approach to purification of a large number of widely different proteins. This standardized protocol can be applied to many samples and can be used in parallel protein purification.",

keywords = "Gel filtration, His-tag affinity purification, His-tag cleavage, Ion exchange chromatography, Parallel protein purification, TEV protease",

author = "Ludmilla Shuvalova",

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year = "2014",

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AB - Structural genomics has increased demands on protein production laboratories to supply large numbers of different proteins in high quantities and qualities in shorter times. Extensive experience with many different technologies and strategies developed and implemented by structural genomics groups have helped identify an optimal approach to purification of a large number of widely different proteins. This standardized protocol can be applied to many samples and can be used in parallel protein purification.

KW - Gel filtration

KW - His-tag affinity purification

KW - His-tag cleavage

KW - Ion exchange chromatography

KW - Parallel protein purification

KW - TEV protease

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Parallel protein purification

Abstract

Keywords

ASJC Scopus subject areas

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