Parallel Three-Dimensional Tracking of Quantum Rods Using Polarization-Sensitive Spectroscopic Photon Localization Microscopy

Biqin Dong, Brian T. Soetikno, Xiangfan Chen, Vadim Backman, Cheng Sun*, Hao F. Zhang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


Semiconductor nanocrystals and their variants are widely used in biological research as fluorescent probes. Their unique characteristics, such as intense brightness, tunable emission properties, and resistance to photobleaching, make them ideal candidates for single-molecule imaging and tracking with localization precision far beyond the diffraction limit. Their fluorescence polarization states and emission spectra can be further utilized to probe changes in their mechanical properties and residing nanoenvironments. We developed a three-dimensional (3D), polarization-sensitive, spectroscopic photon localization microscopy (3D-Polar-SPLM) that enables parallel 3D tracking of individual quantum rods (QRs) while simultaneously capturing their fluorescence spectra and polarization states. Using 3D-Polar-SPLM, we spatially localized individual QRs with a lateral localization precision of 8 nm and an axial localization precision of 35 nm. In addition, we achieved a spectral resolution of 2 nm and a polarization angle measuring precision of 8 degrees. The spectral profile of the fluorescence emission provided a particle-specific signature for identifying individual QRs among the heterogeneous population, which significantly improved the fidelity in parallel 3D tracking of multiple QRs. We envision that this technology will provide new possibilities to reveal the real-time molecular dynamics of biological processes.

Original languageEnglish (US)
Pages (from-to)1747-1752
Number of pages6
JournalACS Photonics
Issue number7
StatePublished - Jul 19 2017


  • polarimetric imaging
  • quantum rods
  • single-particle tracking
  • spectroscopy
  • super-resolution microscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biotechnology
  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering


Dive into the research topics of 'Parallel Three-Dimensional Tracking of Quantum Rods Using Polarization-Sensitive Spectroscopic Photon Localization Microscopy'. Together they form a unique fingerprint.

Cite this