Abstract
Previously, we and others have presented evidence that a calcium second messenger system is involved in the action of parathyroid hormone (PTH) on bone. In the present report, the effects of PTH(1-34) and PTH(3-34)amide treatment on diacylglycerol (DG) in neonatal mouse calvaria are described. PTH(1-34) produced a rapid (within 5 minutes) increase in calvarial incorporation of 3H-arachidonic acid into DG. The effect was maximal at 0.1 nM PTH(1-34), the lowest concentration tested. The 3-34 amide analoge of PTH increased DG to the same extent as PTH(1-34). The effect was maximal at 10 nM PTH(3-34)amide, the lowest concentration tested. These concentrations were lower than those required to elicit maximal effects on bone resorption. In contrast to effects on cyclic AMP, where the 3-34 amide inhibited the increase elicited by PTH, combined treatment of calvaria with PTH(1-34)and PTH(3-34)amide did not inhibit effects on resorption or diacylglycerol.
Original language | English (US) |
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Pages (from-to) | 535-538 |
Number of pages | 4 |
Journal | Hormone and Metabolic Research |
Volume | 23 |
Issue number | 11 |
DOIs | |
State | Published - 1991 |
Keywords
- bone
- calcium
- calvaria
- cyclic AMP
- diacylglycerol
- inositol phosphates
- parathyroid hormone
- parathyroid hormone analogs
- signal transduction
ASJC Scopus subject areas
- Biochemistry, medical
- Endocrinology
- Biochemistry
- Clinical Biochemistry
- Endocrinology, Diabetes and Metabolism