Parathyroid hormone (1-34) and Nleu8,18Tyr34-parathyroid hormone, (3-34) amide increase diacylglycerol in neonatal mouse calvaria

P. M. Stewart, V. M. Stathopoulos, P. H. Stern*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Previously, we and others have presented evidence that a calcium second messenger system is involved in the action of parathyroid hormone (PTH) on bone. In the present report, the effects of PTH(1-34) and PTH(3-34)amide treatment on diacylglycerol (DG) in neonatal mouse calvaria are described. PTH(1-34) produced a rapid (within 5 minutes) increase in calvarial incorporation of 3H-arachidonic acid into DG. The effect was maximal at 0.1 nM PTH(1-34), the lowest concentration tested. The 3-34 amide analoge of PTH increased DG to the same extent as PTH(1-34). The effect was maximal at 10 nM PTH(3-34)amide, the lowest concentration tested. These concentrations were lower than those required to elicit maximal effects on bone resorption. In contrast to effects on cyclic AMP, where the 3-34 amide inhibited the increase elicited by PTH, combined treatment of calvaria with PTH(1-34)and PTH(3-34)amide did not inhibit effects on resorption or diacylglycerol.

Original languageEnglish (US)
Pages (from-to)535-538
Number of pages4
JournalHormone and Metabolic Research
Volume23
Issue number11
DOIs
StatePublished - 1991

Keywords

  • bone
  • calcium
  • calvaria
  • cyclic AMP
  • diacylglycerol
  • inositol phosphates
  • parathyroid hormone
  • parathyroid hormone analogs
  • signal transduction

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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