Abstract
The ionophore, A23187, increased calcium uptake by isolated bone cells in a manner similar to parathyroid hormone. Cellular cyclic AMP was increased after a 30 minute incubation with 0.3 μg/ml A23187; at higher ionophore concentrations (1.0 - 10.0 μg/ml) cyclic AMP was decreased. Resorption of cultured fetal rat long bone also exhibited a biphasic response to the ionophore. With doses of 0.1 - 1.0 μg/ml resorption was increased; higher doses decreased resorption. The data suggest that calcium may be the primary initiator of the action of parathyroid hormone on bone.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1343-1349 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 65 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 18 1975 |
Funding
Supported by NIH grant AM11262
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology