The ionophore, A23187, increased calcium uptake by isolated bone cells in a manner similar to parathyroid hormone. Cellular cyclic AMP was increased after a 30 minute incubation with 0.3 μg/ml A23187; at higher ionophore concentrations (1.0 - 10.0 μg/ml) cyclic AMP was decreased. Resorption of cultured fetal rat long bone also exhibited a biphasic response to the ionophore. With doses of 0.1 - 1.0 μg/ml resorption was increased; higher doses decreased resorption. The data suggest that calcium may be the primary initiator of the action of parathyroid hormone on bone.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Aug 18 1975|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology