Particle concentration fluorescence immunoassay of gentamicin

Pratibha Varma-Nelson, John E. Nelson*, Steven M. Belknap

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

We report the development of a particle concentration fluorescence immunoassay (PCFIA) for gentamicin using commercially available reagents. The solid phase consists of 0.8 μm polystyrene spheres to which gentamicin antibody is noncovalently bound. Fluorescein-labeled gentamicin serves as the tracer. The assay is carried out on 96-well reaction plates and read using an automated instrument. The assay is accurate over the range of gentamicin concentrations commonly encountered in clinical practice, with day-to-day coefficients of variation of 3–7%. As little as 0.01 mg/L of gentamicin can be detected when sample prediction is omitted. PCFIA is compared with Abbott TDX fluorescence polarization immunoassay for the measurement of serum gentamicin concentrations in routine clinical specimens.

Original languageEnglish (US)
Pages (from-to)260-262
Number of pages3
JournalTherapeutic drug monitoring
Volume13
Issue number3
DOIs
StatePublished - May 1991

Keywords

  • Aminoglycosides
  • Fluorescence polarization immunoassay
  • Particle concentration fluorescence immunoassay

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

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