Particle tracking and extended object imaging by interferometric super resolution microscopy

Itay Gdor, Seunghwan Yoo, Xiaolei Wang, Matthew Daddysman, Rosemarie Wilton, Nicola Ferrier, Mark Hereld, Oliver Strides Cossairt, Aggelos K Katsaggelos, Norbert F. Scherer

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking utilizes the information stored in the interference pattern of both the illuminating incoherent light and the emitted light. By periodically shifting the interferometer phase and a phase retrieval algorithm we obtain information that allow localization with sub-2 nm axial resolution at 5 Hz.

Original languageEnglish (US)
Title of host publicationThree-Dimensional and Multidimensional Microscopy
Subtitle of host publicationImage Acquisition and Processing XXV
EditorsTony Wilson, Carol J. Cogswell, Thomas G. Brown
PublisherSPIE
Volume10499
ISBN (Electronic)9781510614833
DOIs
StatePublished - Jan 1 2018
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV 2018 - San Francisco, United States
Duration: Jan 29 2018Jan 31 2018

Other

OtherThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV 2018
CountryUnited States
CitySan Francisco
Period1/29/181/31/18

Fingerprint

Microscopy
Microscopic examination
microscopy
Imaging techniques
Light
Computer-Assisted Image Processing
image resolution
Image resolution
image reconstruction
Image reconstruction
illuminating
Interferometers
retrieval
Microscopes
interferometers
microscopes
interference

Keywords

  • biological imaging
  • interferometric microscope
  • particle tracking
  • super-resolution images

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Gdor, I., Yoo, S., Wang, X., Daddysman, M., Wilton, R., Ferrier, N., ... Scherer, N. F. (2018). Particle tracking and extended object imaging by interferometric super resolution microscopy. In T. Wilson, C. J. Cogswell, & T. G. Brown (Eds.), Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV (Vol. 10499). [1049911] SPIE. https://doi.org/10.1117/12.2291245
Gdor, Itay ; Yoo, Seunghwan ; Wang, Xiaolei ; Daddysman, Matthew ; Wilton, Rosemarie ; Ferrier, Nicola ; Hereld, Mark ; Cossairt, Oliver Strides ; Katsaggelos, Aggelos K ; Scherer, Norbert F. / Particle tracking and extended object imaging by interferometric super resolution microscopy. Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV. editor / Tony Wilson ; Carol J. Cogswell ; Thomas G. Brown. Vol. 10499 SPIE, 2018.
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Gdor, I, Yoo, S, Wang, X, Daddysman, M, Wilton, R, Ferrier, N, Hereld, M, Cossairt, OS, Katsaggelos, AK & Scherer, NF 2018, Particle tracking and extended object imaging by interferometric super resolution microscopy. in T Wilson, CJ Cogswell & TG Brown (eds), Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV. vol. 10499, 1049911, SPIE, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV 2018, San Francisco, United States, 1/29/18. https://doi.org/10.1117/12.2291245

Particle tracking and extended object imaging by interferometric super resolution microscopy. / Gdor, Itay; Yoo, Seunghwan; Wang, Xiaolei; Daddysman, Matthew; Wilton, Rosemarie; Ferrier, Nicola; Hereld, Mark; Cossairt, Oliver Strides; Katsaggelos, Aggelos K; Scherer, Norbert F.

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV. ed. / Tony Wilson; Carol J. Cogswell; Thomas G. Brown. Vol. 10499 SPIE, 2018. 1049911.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Particle tracking and extended object imaging by interferometric super resolution microscopy

AU - Gdor, Itay

AU - Yoo, Seunghwan

AU - Wang, Xiaolei

AU - Daddysman, Matthew

AU - Wilton, Rosemarie

AU - Ferrier, Nicola

AU - Hereld, Mark

AU - Cossairt, Oliver Strides

AU - Katsaggelos, Aggelos K

AU - Scherer, Norbert F.

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N2 - An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking utilizes the information stored in the interference pattern of both the illuminating incoherent light and the emitted light. By periodically shifting the interferometer phase and a phase retrieval algorithm we obtain information that allow localization with sub-2 nm axial resolution at 5 Hz.

AB - An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking utilizes the information stored in the interference pattern of both the illuminating incoherent light and the emitted light. By periodically shifting the interferometer phase and a phase retrieval algorithm we obtain information that allow localization with sub-2 nm axial resolution at 5 Hz.

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KW - interferometric microscope

KW - particle tracking

KW - super-resolution images

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A2 - Cogswell, Carol J.

A2 - Brown, Thomas G.

PB - SPIE

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Gdor I, Yoo S, Wang X, Daddysman M, Wilton R, Ferrier N et al. Particle tracking and extended object imaging by interferometric super resolution microscopy. In Wilson T, Cogswell CJ, Brown TG, editors, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV. Vol. 10499. SPIE. 2018. 1049911 https://doi.org/10.1117/12.2291245