Particle tracking and extended object imaging by interferometric super resolution microscopy

Itay Gdor; Seunghwan Yoo; Xiaolei Wang; Matthew Daddysman; Rosemarie Wilton; Nicola Ferrier; Mark Hereld; Oliver Strides Cossairt; Aggelos K Katsaggelos; Norbert F. Scherer

doi:10.1117/12.2291245

Particle tracking and extended object imaging by interferometric super resolution microscopy

Itay Gdor, Seunghwan Yoo, Xiaolei Wang, Matthew Daddysman, Rosemarie Wilton, Nicola Ferrier, Mark Hereld, Oliver Strides Cossairt, Aggelos K Katsaggelos, Norbert F. Scherer

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking utilizes the information stored in the interference pattern of both the illuminating incoherent light and the emitted light. By periodically shifting the interferometer phase and a phase retrieval algorithm we obtain information that allow localization with sub-2 nm axial resolution at 5 Hz.

Original language	English (US)
Title of host publication	Three-Dimensional and Multidimensional Microscopy
Subtitle of host publication	Image Acquisition and Processing XXV
Editors	Tony Wilson, Carol J. Cogswell, Thomas G. Brown
Publisher	SPIE
ISBN (Electronic)	9781510614833
DOIs	https://doi.org/10.1117/12.2291245
State	Published - Jan 1 2018
Event	Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV 2018 - San Francisco, United States Duration: Jan 29 2018 → Jan 31 2018

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10499
ISSN (Print)	1605-7422

Other

Other	Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV 2018
Country	United States
City	San Francisco
Period	1/29/18 → 1/31/18

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Keywords

biological imaging
interferometric microscope
particle tracking
super-resolution images

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Cite this

Gdor, I., Yoo, S., Wang, X., Daddysman, M., Wilton, R., Ferrier, N., Hereld, M., Cossairt, O. S., Katsaggelos, A. K., & Scherer, N. F. (2018). Particle tracking and extended object imaging by interferometric super resolution microscopy. In T. Wilson, C. J. Cogswell, & T. G. Brown (Eds.), Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV [1049911] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10499). SPIE. https://doi.org/10.1117/12.2291245

Gdor, Itay ; Yoo, Seunghwan ; Wang, Xiaolei ; Daddysman, Matthew ; Wilton, Rosemarie ; Ferrier, Nicola ; Hereld, Mark ; Cossairt, Oliver Strides ; Katsaggelos, Aggelos K ; Scherer, Norbert F. / Particle tracking and extended object imaging by interferometric super resolution microscopy. Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV. editor / Tony Wilson ; Carol J. Cogswell ; Thomas G. Brown. SPIE, 2018. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

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author = "Itay Gdor and Seunghwan Yoo and Xiaolei Wang and Matthew Daddysman and Rosemarie Wilton and Nicola Ferrier and Mark Hereld and Cossairt, {Oliver Strides} and Katsaggelos, {Aggelos K} and Scherer, {Norbert F.}",

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Gdor, I, Yoo, S, Wang, X, Daddysman, M, Wilton, R, Ferrier, N, Hereld, M, Cossairt, OS , Katsaggelos, AK & Scherer, NF 2018, Particle tracking and extended object imaging by interferometric super resolution microscopy. in T Wilson, CJ Cogswell & TG Brown (eds), Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV., 1049911, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10499, SPIE, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV 2018, San Francisco, United States, 1/29/18. https://doi.org/10.1117/12.2291245

Particle tracking and extended object imaging by interferometric super resolution microscopy. / Gdor, Itay; Yoo, Seunghwan; Wang, Xiaolei; Daddysman, Matthew; Wilton, Rosemarie; Ferrier, Nicola; Hereld, Mark; Cossairt, Oliver Strides ; Katsaggelos, Aggelos K; Scherer, Norbert F.

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV. ed. / Tony Wilson; Carol J. Cogswell; Thomas G. Brown. SPIE, 2018. 1049911 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10499).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - Wilton, Rosemarie

AU - Ferrier, Nicola

AU - Hereld, Mark

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AU - Katsaggelos, Aggelos K

AU - Scherer, Norbert F.

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AB - An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking utilizes the information stored in the interference pattern of both the illuminating incoherent light and the emitted light. By periodically shifting the interferometer phase and a phase retrieval algorithm we obtain information that allow localization with sub-2 nm axial resolution at 5 Hz.

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A2 - Cogswell, Carol J.

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Gdor I, Yoo S, Wang X, Daddysman M, Wilton R, Ferrier N et al. Particle tracking and extended object imaging by interferometric super resolution microscopy. In Wilson T, Cogswell CJ, Brown TG, editors, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV. SPIE. 2018. 1049911. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2291245

Access to Document

10.1117/12.2291245