Abstract
An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking utilizes the information stored in the interference pattern of both the illuminating incoherent light and the emitted light. By periodically shifting the interferometer phase and a phase retrieval algorithm we obtain information that allow localization with sub-2 nm axial resolution at 5 Hz.
| Original language | English (US) |
|---|---|
| Title of host publication | Three-Dimensional and Multidimensional Microscopy |
| Subtitle of host publication | Image Acquisition and Processing XXV |
| Editors | Tony Wilson, Carol J. Cogswell, Thomas G. Brown |
| Publisher | SPIE |
| Volume | 10499 |
| ISBN (Electronic) | 9781510614833 |
| DOIs | |
| State | Published - Jan 1 2018 |
| Event | Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV 2018 - San Francisco, United States Duration: Jan 29 2018 → Jan 31 2018 |
Other
| Other | Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV 2018 |
|---|---|
| Country | United States |
| City | San Francisco |
| Period | 1/29/18 → 1/31/18 |
Fingerprint
Keywords
- biological imaging
- interferometric microscope
- particle tracking
- super-resolution images
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Biomaterials
- Radiology Nuclear Medicine and imaging
Cite this
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Particle tracking and extended object imaging by interferometric super resolution microscopy. / Gdor, Itay; Yoo, Seunghwan; Wang, Xiaolei; Daddysman, Matthew; Wilton, Rosemarie; Ferrier, Nicola; Hereld, Mark; Cossairt, Oliver Strides; Katsaggelos, Aggelos K; Scherer, Norbert F.
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV. ed. / Tony Wilson; Carol J. Cogswell; Thomas G. Brown. Vol. 10499 SPIE, 2018. 1049911.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Particle tracking and extended object imaging by interferometric super resolution microscopy
AU - Gdor, Itay
AU - Yoo, Seunghwan
AU - Wang, Xiaolei
AU - Daddysman, Matthew
AU - Wilton, Rosemarie
AU - Ferrier, Nicola
AU - Hereld, Mark
AU - Cossairt, Oliver Strides
AU - Katsaggelos, Aggelos K
AU - Scherer, Norbert F.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking utilizes the information stored in the interference pattern of both the illuminating incoherent light and the emitted light. By periodically shifting the interferometer phase and a phase retrieval algorithm we obtain information that allow localization with sub-2 nm axial resolution at 5 Hz.
AB - An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking utilizes the information stored in the interference pattern of both the illuminating incoherent light and the emitted light. By periodically shifting the interferometer phase and a phase retrieval algorithm we obtain information that allow localization with sub-2 nm axial resolution at 5 Hz.
KW - biological imaging
KW - interferometric microscope
KW - particle tracking
KW - super-resolution images
UR - http://www.scopus.com/inward/record.url?scp=85045320433&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85045320433&partnerID=8YFLogxK
U2 - 10.1117/12.2291245
DO - 10.1117/12.2291245
M3 - Conference contribution
VL - 10499
BT - Three-Dimensional and Multidimensional Microscopy
A2 - Wilson, Tony
A2 - Cogswell, Carol J.
A2 - Brown, Thomas G.
PB - SPIE
ER -