Passaged adult chondrocytes can form engineered cartilage with functional mechanical properties: A canine model

Kenneth W. Ng, Eric G. Lima, Liming Bian, Christopher J. O'Conor, Prakash S. Jayabalan, Aaron M. Stoker, Keiichi Kuroki, Cristi R. Cook, Gerard A. Ateshian, James L. Cook, Clark T. Hung

Research output: Contribution to journalArticlepeer-review

61 Scopus citations


It was hypothesized that previously optimized serum-free culture conditions for juvenile bovine chondrocytes could be adapted to generate engineered cartilage with physiologic mechanical properties in a preclinical, adult canine model. Primary or passaged (using growth factors) adult chondrocytes from three adult dogs were encapsulated in agarose, and cultured in serum-free media with transforming growth factor-β3. After 28 days in culture, engineered cartilage formed by primary chondrocytes exhibited only small increases in glycosaminoglycan content. However, all passaged chondrocytes on day 28 elaborated a cartilage matrix with compressive properties and glycosaminoglycan content in the range of native adult canine cartilage values. A preliminary biocompatibility study utilizing chondral and osteochondral constructs showed no gross or histological signs of rejection, with all implanted constructs showing excellent integration with surrounding cartilage and subchondral bone. This study demonstrates that adult canine chondrocytes can form a mechanically functional, biocompatible engineered cartilage tissue under optimized culture conditions. The encouraging findings of this work highlight the potential for tissue engineering strategies using adult chondrocytes in the clinical treatment of cartilage defects.

Original languageEnglish (US)
Pages (from-to)1041-1051
Number of pages11
JournalTissue Engineering - Part A
Issue number3
StatePublished - Mar 1 2010

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering


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