Passive mechanical properties and related proteins change with botulinum neurotoxin A injection of normal skeletal muscle.

Bryan E. Thacker*, Akihito Tomiya, Jonah B. Hulst, Kentaro P. Suzuki, Shannon N. Bremner, Randy F. Gastwirt, Marion L. Greaser, Richard L. Lieber, Samuel R. Ward

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

The effects of botulinum neurotoxin A on the passive mechanical properties of skeletal muscle have not been investigated, but may have significant impact in the treatment of neuromuscular disorders including spasticity. Single fiber and fiber bundle passive mechanical testing was performed on rat muscles treated with botulinum neurotoxin A. Myosin heavy chain and titin composition of single fibers was determined by gel electrophoresis. Muscle collagen content was determined using a hydroxyproline assay. Neurotoxin-treated single fiber passive elastic modulus was reduced compared to control fibers (53.00 kPa vs. 63.43 kPa). Fiber stiffness and slack sarcomere length were also reduced compared to control fibers and myosin heavy chain composition shifted from faster to slower isoforms. Average titin molecular weight increased 1.77% after treatment. Fiber bundle passive elastic modulus increased following treatment (168.83 kPa vs. 75.14 kPa). Bundle stiffness also increased while collagen content per mass of muscle tissue increased 38%. Injection of botulinum neurotoxin A produces an effect on the passive mechanical properties of normal muscle that is opposite to the changes observed in spastic muscles.

Original languageEnglish (US)
Pages (from-to)497-502
Number of pages6
JournalJournal of orthopaedic research : official publication of the Orthopaedic Research Society
Volume30
Issue number3
DOIs
StatePublished - Mar 2012

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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