Widespread application of micro-fluidic systems has been hindered by the challenge of repeatable, accurate interfacing between integrated channels or wells and external loading, detecting, or stimulating devices. We are developing a repeatable, accurate, 100-port micro-fluidic connector to interface to a 100 capillary array. The capillaries are passively aligned for connection with a combination of precision guide pins and conical tapered holes, and sealed with a PDMS layer after insertion. Using the connector device, an array of 100 loosely-assembled capillaries with 1 mm spacing can be aligned to 20 μm radial accuracy and high repeatability, with only 5% failure after 10,000 insertions. We have confirmed electrical and fluidic connectivity as well. This device can be simply manufactured and adapted for numerous micro-fluidic interface applications.