Pathogenesis of NOD diabetes is initiated by reactivity to the insulin B chain 9-23 epitope and involves functional epitope spreading

Suchitra Prasad, Adam P. Kohm, Jeffrey S. McMahon, Xunrong Luo, Stephen D. Miller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

Type 1 diabetes (T1D) is mediated by destruction of pancreatic β-cells by CD4 and CD8 T cells specific for epitopes on numerous diabetogenic autoantigens resulting in loss of glucose homeostasis. Employing antigen-specific tolerance induced by i.v. administration of syngeneic splenocytes ECDI cross-linked to various diabetogenic antigens/epitopes (Ag-SP), we show that epitope spreading plays a functional role in the pathogenesis of T1D in NOD mice. Specifically, Ag-SP coupled with intact insulin, Ins B9-23 or Ins B15-23, but not GAD65509-528, GAD65524-543 or IGRP206-214, protected 4-6 week old NOD mice from the eventual development of clinical disease; infiltration of immune cells to the pancreatic islets; and blocked the induction of DTH responses in a Treg-dependent, antigen-specific manner. However, tolerance induction in 19-21 week old NOD mice was effectively accomplished only by Ins-SP, suggesting Ins B9-23 is a dominant initiating epitope, but autoimmune responses to insulin epitope(s) distinct from Ins B9-23 emerge during disease progression.

Original languageEnglish (US)
Pages (from-to)347-353
Number of pages7
JournalJournal of Autoimmunity
Volume39
Issue number4
DOIs
StatePublished - Dec 2012

Keywords

  • Autoimmune disease
  • Diabetes
  • Epitope spreading
  • Immunoregulation
  • Insulin
  • Pancreatic β-cells
  • T cell activation
  • T lymphocytes
  • Tolerance

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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