TY - JOUR
T1 - Pattern of prevalent Hepatitis B virus genotypes in Zaria, Nigeria
AU - Ahmad, Abdurrahman Elfulaty
AU - Bakari, Adamu Girei
AU - Musa, Bolanle Olufunke Priscilla
AU - Mustapha, Shettima Kagu
AU - Jamoh, Bello Yusuf
AU - Abdullahi, Idris Nasir
AU - Tahir, Mohammed Ibrahim
AU - Olatunji, Abdulqadri Olarenwaju
AU - Maishanu, Sumayya Hamza
AU - Suleiman, Ahmed Babangida
AU - Tolulope, Afolaranmi
AU - Hawkins, Claudia
AU - Sagay, Atiene Solomon
AU - Zoakah, Ayuba
AU - Olayinka, Adebola Tolulope
PY - 2019/4/1
Y1 - 2019/4/1
N2 - Background: Hepatitis B virus (HBV) is hyperendemic in Nigeria. Available literature reveal genotype E as being predominant in West Africa. This study aimed at identifying the current pattern and prevalent genotypes of HBV in Zaria, Nigeria. Materials and Methods: Four millilitre of blood was collected in ethylenediaminetetraacetic acid-container from each of 165 HBV surface antigen-positive participants recruited purposively from the gastroenterology clinic from May to August, 2017. Plasma was separated and frozen at -20°C till analysis. Multiplex-nested polymerase chain reaction using type-specific primers was used to identify the various HBV genotypes. Results: Median (and interquartile range) age of the participants was 31.0 (25.5-39.0) years, with males constituting 107 (64.8%). Majority (83.6%) of the samples analysed were HBV-DNA-positive with 82.6% of the HBV-DNA-positive samples being mixed genotype infections. Irrespective of mode of occurrence, five HBV genotypes were identified with HBV/E (97.1%) being the most predominant, followed by HBV/B (82.6%), HBV/A (24.6%), then HBV/C (17.4%), while HBV/D (0.7%) was the least prevalent. Conclusion: In most (99.1%) of the mixed-infection were a combination of genotype E, the predominant genotype, with other genotypes predominantly genotype B. HBV genotypes E, B, A, C and D are the prevalent genotypes in Zaria, Nigeria, as they occur in single genotype and in mixed-genotypes pattern.
AB - Background: Hepatitis B virus (HBV) is hyperendemic in Nigeria. Available literature reveal genotype E as being predominant in West Africa. This study aimed at identifying the current pattern and prevalent genotypes of HBV in Zaria, Nigeria. Materials and Methods: Four millilitre of blood was collected in ethylenediaminetetraacetic acid-container from each of 165 HBV surface antigen-positive participants recruited purposively from the gastroenterology clinic from May to August, 2017. Plasma was separated and frozen at -20°C till analysis. Multiplex-nested polymerase chain reaction using type-specific primers was used to identify the various HBV genotypes. Results: Median (and interquartile range) age of the participants was 31.0 (25.5-39.0) years, with males constituting 107 (64.8%). Majority (83.6%) of the samples analysed were HBV-DNA-positive with 82.6% of the HBV-DNA-positive samples being mixed genotype infections. Irrespective of mode of occurrence, five HBV genotypes were identified with HBV/E (97.1%) being the most predominant, followed by HBV/B (82.6%), HBV/A (24.6%), then HBV/C (17.4%), while HBV/D (0.7%) was the least prevalent. Conclusion: In most (99.1%) of the mixed-infection were a combination of genotype E, the predominant genotype, with other genotypes predominantly genotype B. HBV genotypes E, B, A, C and D are the prevalent genotypes in Zaria, Nigeria, as they occur in single genotype and in mixed-genotypes pattern.
KW - Genotypes
KW - Nigeria
KW - Zaria
KW - hepatitis B Virus
KW - mixed-infection
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U2 - 10.4103/npmj.npmj_59_19
DO - 10.4103/npmj.npmj_59_19
M3 - Article
C2 - 31187746
AN - SCOPUS:85067820533
SN - 1117-1936
VL - 26
SP - 80
EP - 86
JO - The Nigerian postgraduate medical journal
JF - The Nigerian postgraduate medical journal
IS - 2
ER -
