We developed a patterned-illumination second harmonic generation (PI-SHG) microscopy, which combines the principle of structured illumination reconstruction with SHG microscopy for label-free super-resolution imaging. We confirmed that PI-SHG microscopy can achieve 1.59-time resolution improvement compared to conventional SHG microscopy by imaging nanowire samples. We further demonstrated three-dimensional PI-SHG microscopy in imaging ex vivo collagen fibrils in rat scleras.
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics