Patterned-illumination second harmonic generation microscopy of collagen fibrils in rat scleras

Ben E. Urban, Biqin Dong, Xian Zhang, Huili Yang, Hao F. Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We developed a patterned-illumination second harmonic generation (PI-SHG) microscopy, which combines the principle of structured illumination reconstruction with SHG microscopy for label-free super-resolution imaging. We confirmed that PI-SHG microscopy can achieve 1.59-time resolution improvement compared to conventional SHG microscopy by imaging nanowire samples. We further demonstrated three-dimensional PI-SHG microscopy in imaging ex vivo collagen fibrils in rat scleras.

Original languageEnglish (US)
Pages (from-to)5190-5193
Number of pages4
JournalOptics Letters
Volume43
Issue number21
DOIs
StatePublished - Nov 1 2018

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Fingerprint Dive into the research topics of 'Patterned-illumination second harmonic generation microscopy of collagen fibrils in rat scleras'. Together they form a unique fingerprint.

Cite this