Patterns of neurofilament stain in the spiral ganglion of the developing and adult mouse

R. Romand, H. Sobkowicz*, M. Emmerling, D. Whitlon, D. Dahl

*Corresponding author for this work

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The objective of the study was to identify neurofilament-positive cells and their projections in the intact spiral ganglia of the mouse. One polyclonal and three monoclonal antibodies against neurofilament triplet subunits NF 68 K, 160 K and 200 K were used. In the newborn mouse most of the spiral neurons and their processes stain positively, although the perikaryal stain is very light. During early postnatal development, some cells show a selective intense stain. The progressive myelination of the neuronal processes further restricts the stain to a small neuronal population of positive perikarya and to their nonmyelinated fibers. This pattern of stainability implies that the neurofilament-positive cells are compatible with the type II spiral neurons. The stain reveals two populations of spiral neurons: 1) the cells which are scattered within the ganglion and show a bipolar distribution of fibers; and 2) the cells that form an interrupted chain along the intraganglionic bundle. The latter cells are also bipolar, but their peripheral processes join the intraganglionic bundle for varying distances before reaching the radial bundles. The identification of selective groupings of filamentous nonmyelinated cells in the corresponding location in different mammals is discussed. In conclusion, the use of neurofilament antibodies in staining of the intact spiral neurons permitted us to identify a distinct cell population of neurofilament-positive nonmyelinated nerve cells located along and projecting (at least partly) into the intraganglionic bundle.

Original languageEnglish (US)
Pages (from-to)119-125
Number of pages7
JournalHearing Research
Volume49
Issue number1-3
DOIs
StatePublished - Jan 1 1990

Fingerprint

Spiral Ganglion
Intermediate Filaments
Coloring Agents
Neurons
Population
Ganglia
Mammals
Monoclonal Antibodies
Staining and Labeling
Light
Antibodies

Keywords

  • Immunocytochemistry
  • Mouse
  • Neurofilaments
  • Neurons, type II
  • Spiral ganglion

ASJC Scopus subject areas

  • Sensory Systems

Cite this

Romand, R. ; Sobkowicz, H. ; Emmerling, M. ; Whitlon, D. ; Dahl, D. / Patterns of neurofilament stain in the spiral ganglion of the developing and adult mouse. In: Hearing Research. 1990 ; Vol. 49, No. 1-3. pp. 119-125.
@article{00e3e4532a7c4bd4b0e7fc7c26d475de,
title = "Patterns of neurofilament stain in the spiral ganglion of the developing and adult mouse",
abstract = "The objective of the study was to identify neurofilament-positive cells and their projections in the intact spiral ganglia of the mouse. One polyclonal and three monoclonal antibodies against neurofilament triplet subunits NF 68 K, 160 K and 200 K were used. In the newborn mouse most of the spiral neurons and their processes stain positively, although the perikaryal stain is very light. During early postnatal development, some cells show a selective intense stain. The progressive myelination of the neuronal processes further restricts the stain to a small neuronal population of positive perikarya and to their nonmyelinated fibers. This pattern of stainability implies that the neurofilament-positive cells are compatible with the type II spiral neurons. The stain reveals two populations of spiral neurons: 1) the cells which are scattered within the ganglion and show a bipolar distribution of fibers; and 2) the cells that form an interrupted chain along the intraganglionic bundle. The latter cells are also bipolar, but their peripheral processes join the intraganglionic bundle for varying distances before reaching the radial bundles. The identification of selective groupings of filamentous nonmyelinated cells in the corresponding location in different mammals is discussed. In conclusion, the use of neurofilament antibodies in staining of the intact spiral neurons permitted us to identify a distinct cell population of neurofilament-positive nonmyelinated nerve cells located along and projecting (at least partly) into the intraganglionic bundle.",
keywords = "Immunocytochemistry, Mouse, Neurofilaments, Neurons, type II, Spiral ganglion",
author = "R. Romand and H. Sobkowicz and M. Emmerling and D. Whitlon and D. Dahl",
year = "1990",
month = "1",
day = "1",
doi = "10.1016/0378-5955(90)90099-B",
language = "English (US)",
volume = "49",
pages = "119--125",
journal = "Hearing Research",
issn = "0378-5955",
publisher = "Elsevier",
number = "1-3",

}

Patterns of neurofilament stain in the spiral ganglion of the developing and adult mouse. / Romand, R.; Sobkowicz, H.; Emmerling, M.; Whitlon, D.; Dahl, D.

In: Hearing Research, Vol. 49, No. 1-3, 01.01.1990, p. 119-125.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Patterns of neurofilament stain in the spiral ganglion of the developing and adult mouse

AU - Romand, R.

AU - Sobkowicz, H.

AU - Emmerling, M.

AU - Whitlon, D.

AU - Dahl, D.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - The objective of the study was to identify neurofilament-positive cells and their projections in the intact spiral ganglia of the mouse. One polyclonal and three monoclonal antibodies against neurofilament triplet subunits NF 68 K, 160 K and 200 K were used. In the newborn mouse most of the spiral neurons and their processes stain positively, although the perikaryal stain is very light. During early postnatal development, some cells show a selective intense stain. The progressive myelination of the neuronal processes further restricts the stain to a small neuronal population of positive perikarya and to their nonmyelinated fibers. This pattern of stainability implies that the neurofilament-positive cells are compatible with the type II spiral neurons. The stain reveals two populations of spiral neurons: 1) the cells which are scattered within the ganglion and show a bipolar distribution of fibers; and 2) the cells that form an interrupted chain along the intraganglionic bundle. The latter cells are also bipolar, but their peripheral processes join the intraganglionic bundle for varying distances before reaching the radial bundles. The identification of selective groupings of filamentous nonmyelinated cells in the corresponding location in different mammals is discussed. In conclusion, the use of neurofilament antibodies in staining of the intact spiral neurons permitted us to identify a distinct cell population of neurofilament-positive nonmyelinated nerve cells located along and projecting (at least partly) into the intraganglionic bundle.

AB - The objective of the study was to identify neurofilament-positive cells and their projections in the intact spiral ganglia of the mouse. One polyclonal and three monoclonal antibodies against neurofilament triplet subunits NF 68 K, 160 K and 200 K were used. In the newborn mouse most of the spiral neurons and their processes stain positively, although the perikaryal stain is very light. During early postnatal development, some cells show a selective intense stain. The progressive myelination of the neuronal processes further restricts the stain to a small neuronal population of positive perikarya and to their nonmyelinated fibers. This pattern of stainability implies that the neurofilament-positive cells are compatible with the type II spiral neurons. The stain reveals two populations of spiral neurons: 1) the cells which are scattered within the ganglion and show a bipolar distribution of fibers; and 2) the cells that form an interrupted chain along the intraganglionic bundle. The latter cells are also bipolar, but their peripheral processes join the intraganglionic bundle for varying distances before reaching the radial bundles. The identification of selective groupings of filamentous nonmyelinated cells in the corresponding location in different mammals is discussed. In conclusion, the use of neurofilament antibodies in staining of the intact spiral neurons permitted us to identify a distinct cell population of neurofilament-positive nonmyelinated nerve cells located along and projecting (at least partly) into the intraganglionic bundle.

KW - Immunocytochemistry

KW - Mouse

KW - Neurofilaments

KW - Neurons, type II

KW - Spiral ganglion

UR - http://www.scopus.com/inward/record.url?scp=0025119303&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025119303&partnerID=8YFLogxK

U2 - 10.1016/0378-5955(90)90099-B

DO - 10.1016/0378-5955(90)90099-B

M3 - Article

VL - 49

SP - 119

EP - 125

JO - Hearing Research

JF - Hearing Research

SN - 0378-5955

IS - 1-3

ER -