Pax3:Foxc2 Reciprocal Repression in the Somite Modulates Muscular versus Vascular Cell Fate Choice in Multipotent Progenitors

Mounia Lagha, Silvia Brunelli, Graziella Messina, Ana Cumano, Tsutomu Kume, Frédéric Relaix, Margaret E. Buckingham*

*Corresponding author for this work

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

Maintenance of multipotency and how cells exit this state to adopt a specific fate are central questions in stem cell biology. During vertebrate development, multipotent cells of the dorsal somite, the dermomyotome, give rise to different lineages such as vascular smooth and skeletal muscle, regulated by the transcription factors Foxc2 and Pax3, respectively. Here we show reciprocal inhibition between Pax3 and Foxc2 in the mouse embryo. Using both genetic approaches and manipulation of external signals in somite explants, we demonstrate that the Pax3:Foxc2 ratio modulates myogenic versus vascular cell fates. This provides insight into how cell fate choices are orchestrated by these lineage genes in the dermomyotome.

Original languageEnglish (US)
Pages (from-to)892-899
Number of pages8
JournalDevelopmental Cell
Volume17
Issue number6
DOIs
Publication statusPublished - Dec 15 2009

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Keywords

  • DEVBIO

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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