Abstract
In a yeast two-hybrid screen for cellular factors that could interact with human immunodeficiency virus type 1 (HIV-1) Gag protein, we identified PDZD8 and confirmed the interaction by coimmunoprecipitation (co-IP). PDZD8 overexpression promoted the initiation of reverse transcription and increased infection by pseudotyped retroviruses independent of the route of viral entry, while transient knockdown of endogenous levels decreased HIV-1 infection. A mutant of PDZD8 lacking a predicted coiled-coil domain in its Gag-interacting region failed to bind Gag and promote HIV-1 infection, identifying the domain of PDZD8 required for mediating these effects. As such, we identify PDZD8 as a novel positive mediator of retroviral infection.
Original language | English (US) |
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Pages (from-to) | 8990-8995 |
Number of pages | 6 |
Journal | Journal of virology |
Volume | 84 |
Issue number | 17 |
DOIs | |
State | Published - Sep 2010 |
ASJC Scopus subject areas
- Insect Science
- Virology
- Microbiology
- Immunology