PDZD8 is a novel gag-interacting factor that promotes retroviral infection

Matthew S. Henning, Scott G. Morham, Stephen P. Goff, Mojgan H. Naghavi

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

In a yeast two-hybrid screen for cellular factors that could interact with human immunodeficiency virus type 1 (HIV-1) Gag protein, we identified PDZD8 and confirmed the interaction by coimmunoprecipitation (co-IP). PDZD8 overexpression promoted the initiation of reverse transcription and increased infection by pseudotyped retroviruses independent of the route of viral entry, while transient knockdown of endogenous levels decreased HIV-1 infection. A mutant of PDZD8 lacking a predicted coiled-coil domain in its Gag-interacting region failed to bind Gag and promote HIV-1 infection, identifying the domain of PDZD8 required for mediating these effects. As such, we identify PDZD8 as a novel positive mediator of retroviral infection.

Original languageEnglish (US)
Pages (from-to)8990-8995
Number of pages6
JournalJournal of virology
Volume84
Issue number17
DOIs
StatePublished - Sep 2010

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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