Peptide antisense nanoparticles

Pinal C. Patel, David A. Giljohann, Dwight S. Seferos, Chad A. Mirkin

Research output: Contribution to journalArticle

83 Scopus citations

Abstract

We have designed a heterofunctionalized nanoparticle conjugate consisting of a 13-nm gold nanoparticle (Au NP) containing both antisense oligonucleotides and synthetic peptides. The synthesis of this conjugate is accomplished by mixing thiolated oligonucleotides and cysteine-terminated peptides with gold nanoparticles in the presence of salt, which screens interactions between biomolecules, yielding a densely functionalized nanomaterial. By controlling the stoichiometry of the components in solution, we can control the surface loading of each biomolecule. The conjugates are prepared easily and show perinuclear localization and an enhanced gene regulation activity when tested in a cellular model. This heterofunctionalized structure represents a new strategy for preparing nanomaterials with potential therapeutic applications.

Original languageEnglish (US)
Pages (from-to)17222-17226
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number45
DOIs
StatePublished - Nov 11 2008

Keywords

  • Gene regulation
  • Gold nanoparticles
  • Heterofunctional

ASJC Scopus subject areas

  • General

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