Per-6-substituted β-cyclodextrin libraries inhibit formation of β-amyloid-peptide (Aβ)-derived, soluble oligomers

Jiaxin Yu, Lara Bakhos, Lei Chang, Mark J. Holterman, William L. Klein, Duane L. Venton*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Alzheimer's disease is the most common cause of dementia in older individuals with compelling evidence favoring neuron dysfunction and death triggered by assembled forms of Aβ1-42. While large neurotoxic amyloid fibrils have been known for years, recent studies show that soluble protofibril and Aβ1-42-derived diffusible ligands (ADDLs) may also be involved in neurotoxicity. In the present work, dot-blot immunoassays discriminating ADDLs from monomers were used to screen libraries of per-substituted β-cyclodextrin (β-CD) derivatives for inhibition of ADDLs formation. Libraries were prepared from per-6-iodo-β-CD by treatment with various amine nucleophiles. The most active library tested (containing >2000 derivatives) was derived from imidazole, N, N-dimethylethylenediamine and furfurylamine, which at 10 μM total library, inhibited ADDLs formation (10 nM Aβ1-42) over a period of 4 hours. The latter was confirmed by a western blot assay showing decreased amounts of the initially formed Aβ1-42 tetramer. These preliminary experiments suggest that derivatized forms of β-CD can interfere with the oligomerization process of Aβ1-42.

Original languageEnglish (US)
Pages (from-to)51-55
Number of pages5
JournalJournal of Molecular Neuroscience
Volume19
Issue number1-2
DOIs
StatePublished - Jan 1 2002

Keywords

  • ADDLs
  • Alzheimer's
  • Beta amyloid
  • Beta cyclodextrin
  • Combinatorial chemistry

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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