TY - JOUR
T1 - Performance of Xpert MTB/RIF in comparison with light-emitting diode-fluorescence microscopy and culture for detecting tuberculosis in pulmonary and extrapulmonary specimens in Bamako, Mali
AU - Combo Georges, Togo
AU - Aissata, Traore
AU - Fatimata, Diallo
AU - Abou, Coulibaly
AU - Gagni, Coulibaly
AU - Moise, Somboro
AU - Boureima, Degoga
AU - Amadou, Somboro
AU - Baye, Drame
AU - Price Polycarpe, Dembele
AU - Bourahima, Kone
AU - Moumine, Sanogo
AU - Dit Sadio, Sarro
AU - Ousmane, Kodio
AU - Bocar, Baya
AU - Mohamed, Tolofoudie
AU - Boubakar, Traore
AU - Sidy, Bane
AU - Mamoudou, Maiga
AU - Michael, Belson
AU - Seydou, Doumbia
AU - Bassirou, Diarra
AU - Ibrahima, I.
AU - Souleymane, Diallo
N1 - Publisher Copyright:
© 2020 Wolters Kluwer Medknow Publications. All rights reserved.
PY - 2020/10/1
Y1 - 2020/10/1
N2 - Background: The diagnosis of tuberculosis (TB) has mostly been relied on a long-used method called sputum smear microscopy. In 2010, Xpert MTB/RIF assay was approved by the World Health Organization for simultaneous TB diagnosis and detection of resistance. Our current study was undertaken to compare the diagnostic performance of Xpert MTB/RIF assay to auramine staining-based light-emitting diode-Fluorescence Microscopy (LED-FM) considering culture as the gold standard method for pulmonary and extrapulmonary TB. Method: Pulmonary and extrapulmonary specimens of suspected TB patients were examined in this study. From January 2016 to June 2019, sputum, urine, superficial swabs, gastric aspirates, and pleural infusion specimens were collected from new and previously treated TB individuals. Specimens were examined using Xpert MTB/RIF, LED-FM, and Mycobacterium culture techniques to evaluate their performance. Results: A total of 697 suspected TB samples were included in this analysis, and of these, 469 (67.29%) were positive for all three used methods. The overall sensitivities, specificities, and positive and negative predictive values were 99.6%, 62.0%, 88.4%, and 98.2% for Xpert MTB/RIF and 88.0%, 95.6%, 99.0%, and 60.7% for LED-FM, respectively, compared to culture method. Conclusion: The sensitivity of Xpert MTB/RIF assay was observed to be higher than the LED-FM method, thus suggesting this molecular technique as a promising tool for the diagnosis of pulmonary and extrapulmonary TB, which will help in the management of TB infections in developing countries such as Mali.
AB - Background: The diagnosis of tuberculosis (TB) has mostly been relied on a long-used method called sputum smear microscopy. In 2010, Xpert MTB/RIF assay was approved by the World Health Organization for simultaneous TB diagnosis and detection of resistance. Our current study was undertaken to compare the diagnostic performance of Xpert MTB/RIF assay to auramine staining-based light-emitting diode-Fluorescence Microscopy (LED-FM) considering culture as the gold standard method for pulmonary and extrapulmonary TB. Method: Pulmonary and extrapulmonary specimens of suspected TB patients were examined in this study. From January 2016 to June 2019, sputum, urine, superficial swabs, gastric aspirates, and pleural infusion specimens were collected from new and previously treated TB individuals. Specimens were examined using Xpert MTB/RIF, LED-FM, and Mycobacterium culture techniques to evaluate their performance. Results: A total of 697 suspected TB samples were included in this analysis, and of these, 469 (67.29%) were positive for all three used methods. The overall sensitivities, specificities, and positive and negative predictive values were 99.6%, 62.0%, 88.4%, and 98.2% for Xpert MTB/RIF and 88.0%, 95.6%, 99.0%, and 60.7% for LED-FM, respectively, compared to culture method. Conclusion: The sensitivity of Xpert MTB/RIF assay was observed to be higher than the LED-FM method, thus suggesting this molecular technique as a promising tool for the diagnosis of pulmonary and extrapulmonary TB, which will help in the management of TB infections in developing countries such as Mali.
KW - Bamako
KW - Mali
KW - Xpert MTB/RIF
KW - culture
KW - light-emitting diode fluorescent microscopy
UR - http://www.scopus.com/inward/record.url?scp=85098128128&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85098128128&partnerID=8YFLogxK
U2 - 10.4103/ijmy.ijmy-171-20
DO - 10.4103/ijmy.ijmy-171-20
M3 - Article
C2 - 33323655
AN - SCOPUS:85098128128
SN - 2212-5531
VL - 9
SP - 397
EP - 404
JO - International Journal of Mycobacteriology
JF - International Journal of Mycobacteriology
IS - 4
ER -
